It's not that hard to open a hospital in another world, right?

Chapter 228 Gretel Plague Company Nordmark Arrives! (Mr. Chun's lord adds 7)

Facing the bloody facts (some rabbits really spit out pink bubbles), Gretel was finally sent down the mountain.The old man in overalls sent him to the station of the little train, following every step, reluctant to part:
"You can do it quickly! We have already made 30 high-precision microscopes!"

"The ones that were magnified by 100 times were all sold, and the ones with larger multiples were too expensive and no one would buy them. Just wait for your thesis to come out, and go to the gang of Black Crow Swamp guys!"

Gretel: "..." You must speak with conscience!What is a pit!You translate for me, what is a **** pit!

Given the size of bacteria, they can only be seen under a microscope!Only by increasing the magnification to 1000 times can the characteristics of bacteria be identified!Only through bacterial staining, various inspection methods, and animal experiments can we determine which bacteria caused which disease!

Isn't it the best of both worlds that I give them the research tools, pave the way for a while, and make some money for myself?He also said that there is a pit, where is the pit!
When he went there, he had a carriage. When he came back, several carriages came to the laboratory one after another:

An alchemist specially sent him a customized shadowless lamp, which was installed in a separate dissection room and debugged for more than half an hour;

Another alchemist brought two space bags, one bottle and one bottle, and put down all the dyes that can be found on the market and in the laboratory, and all liquids and solids that can be dyed, as well as alcohol, sulfuric acid, and nitric acid ... a whole lot of drugs;

The third alchemist who came to the door was ordered to settle with Gretel: the shadowless lamp has not been sold, and the microscope is settled with contribution points. In addition, Gretel got a full 87 gold coins and 5 silver coins. 9 Copper Coins - A share of sales of pencils.

It must be said that the production and sales capabilities of the Alchemy Union are really outstanding.

Damn!

There is money again!

There are gold coins again!
You can make it again, no, you can have money to make Motui ghosts and use gold coins to work!
Hearing the sound of jingling coins, Gretel beamed with joy.

He had just sorted things out here, and soon the fourth, fifth, and sixth cars came—this time it was not a carriage, but a flatbed cart pulled by human power.A cart full of rabbits, a cart of feed for rabbits, a cart of seaweed and sea stone flowers, with a few large pieces of beef and mutton on it, bright in color, it looks like it was just killed today.

Ah...how much does it cost...

Bernard looked beside him with bright eyes.Gretel glanced at him, pursed his lips, and took out his wallet in pain.My gold coins, my little money, I'm saying goodbye before it's hot...

"This...you don't need to pay for it, you don't need to pay for it." The boss who followed the escort hurried forward, nodded and bowed, and waved his hands again and again: "The council has already paid!"

Gretel turned to look at him.The boss was wearing a bronze velvet jacket, the neckline and hem were worn white, and a small hexagram was embroidered with blue silk thread on the hidden part of the cuffs.Throwing an identification technique casually, a phantom appeared on the six-pointed star, obviously a supplier authorized by the council.

Gretel was slightly relieved.Looking further down, the high leather boots under the boss's feet were splashed with mud, and there was also a fishy smell. It was obvious that seaweed was transported from the port area and directly transported here.He ran from the back of the second carriage to the front, nodded and bowed, and handed over a double-folded list:

"My lord, check it out. If there is no problem with the quantity, I will go to the council to settle the bill."

Gretel glanced, and saw a black skull flashing on the lower right corner of the list...

He opens the list.A wisp of black smoke rose from the mark, and the upper and lower jaws of the skull opened and closed, making a dry mechanical sound:

"Little Gretel, hurry up, the plague branch is waiting!"

Gretel: "..." No, this project has been transferred to Black Crow Swamp?

The Alchemy Guild is so quick!Or do you not want to pay, just use the previous thesis to fool Black Crow Swamp into the sponsor, and put up the project list?

Either way, it's nice to have someone pay.Immediately, Gretel's mouth didn't deflate, his heart didn't hurt, and his head straightened up.He scanned the list at a glance, turned his head, and commanded the barbarians:

"Bernard, go and count the rabbits! There are 20 rabbits in the car, see if the number is correct, and if all of them are healthy!"

The experimental animals are in place, the experimental equipment is in place, and various experimental materials-such as various dyes for bacterial staining, sea stone flowers for agar, beef and mutton for broth culture medium...are also in place one by one.Gretel retreated there, happily pouring his penicillin.

Well, the environment in the laboratory is really good... No worries about food, clothes, and wind and rain.Except for the noise in the laboratory and the sound of horseshoes passing by outside from time to time, there is basically nothing to criticize.

Of course, the sound of horseshoes is also because the city of Nevis has expanded the security area to include his laboratory, so it feels very safe...

"Ah... experiment, experiment, experiment..."

Doing bacterial culture experiments is actually very boring.In particular, you have to cook the medium yourself, dye the color yourself, iodine or something, start by burning the seaweed yourself...

If you are just a science enthusiast, do an experiment for entertainment, then forget it.Making results can be regarded as adding a sense of accomplishment, and it doesn't matter if you can't make results, anyway, you have already enjoyed the process.

However, Mr. Gretel had to rely on these crude experimental conditions to really produce results...

"Ah - there are still 500 samples to be dyed~~~"

In the empty laboratory where he was the only one, Gretel let out a heartfelt wail.

Bacterial staining is a technical job, but also a job that depends on materials.In Hartland City, Gretel searched all the dyes in the city, tried everything, but only found a dye extracted from wood, which could dye the cells into a light blue.

The reserve of the Magic Council is much richer than that of the small city of Hartland. There are three to four hundred kinds of dyes sent to the laboratory - counting the use of acids, alkalis, and various chemicals. This workload is becoming more and more terrifying to the point that people dare not look directly at it.

But such a large pile of materials, apart from increasing Gretel's fatigue, did not produce much results - except for a red dye, the others either could not be dyed, or could be washed off with water.

"I want gentian violet! Crystal violet!" Gretel flipped the table angrily.There's iodine, there's red dye, give him gentian violet, and he'll be able to tell the difference between gram positives and gram negatives!However, the premise of everything is to let him get gentian violet!
Gretel wrote angrily, and pressed the sharpened pencil against the white paper, poking two holes in a row.His demand list was brought back by the supplier boss. On the third day, a magician made a special trip to the door and delivered a space bag of materials:

"This is a new purple dye we found. This is conch purple, this is mountain violet, this is lac insect powder, this is comfrey purple... This is the solution extracted from gentian flower. Although it is not purple, we also I brought it here, by the way, there are two bundles of gentian flowers here... And there is no gentian, but here are different kinds of snake gall and fish gall, you can use it..."

Gretel: "T_T..."

How did the senior Gram find gentian violet?What's the chemical formula for this thing?How was it synthesized?
Gretel searched hard, vaguely remembered that this thing was a mixture of tetramethylpararosaniline chloride, pentamethylpararosaniline chloride, and hexamethylpararosaniline chloride...

The name alone is enough of a mouthful.If it wasn't for the memory power that came with traveling once, he wouldn't even be able to recite the official name.As for how to make this thing, Gretel said that he is not a chemical major... Cough, did the pharmacy major next door do gentian violet?
I'm so hard, I have nothing...

The only solution to worry is anatomy.Gretel spawned, purified, spawned, and purified again the bacteria he made, and tossed them on the rabbits in different ways:
Mix it in the feed and feed it to the rabbits;

Let the rabbits rub their nasal cavity and respiratory tract;

Cut a small cut in the rabbit's skin, and apply it to the skin wound...

Then, observe, measure signs, and record the course of the disease.When the rabbit is dead, or half-dead and dying, kill it again, and cut open the organs one by one to observe the damage of the organs.

Fortunately, he can still dissect, and he can dissect more than a dozen or twenty rabbits every day, so as to maintain his hand feeling... He will think that he is not a surgeon anymore and go to the laboratory department after cultivating, cultivating, dyeing and dyeing every day...

"Hmm... Rabbit A03, the whole body is dehydrated, the skin is dry and inelastic, the subcutaneous tissue and muscles are dehydrated, the heart, liver, and spleen are shrunk due to dehydration... the small intestine is obviously edema... the kidney is necrotic..."

Gretel took a look and made a note.After writing everything, I quickly glanced at the previous records:

"Feeding at 21:05 a.m. on the 2st. The onset began at 37:[-] p.m.... The excrement was like rice slop... The excrement was smeared and the results were as follows..."

In the corner of the record book, there is also a hand-painted schematic diagram. The bacteria on it are like commas one by one, and like a group of tadpoles, they are arranged in groups and move lively.

Very good, combined with the shape of the bacteria, the course of the disease, the time of death and the results of the autopsy, it is basically certain that this thing is [-]% likely to be Vibrio cholerae.

Gretel smiled contentedly.Following the numbers on the record book, he carefully took out two petri dishes and kept them separately.One is used to give an account to the Necromancer School, and the other one is reserved for cholera vaccine——

Born in this world where you don't need anything, it all depends on your physique and healing skills, and there are few vaccines available, so you always feel very low in your sense of security.

Looking around the locker again, Gretel's smile became even lighter.Suspected Staphylococcus aureus, the culture is completed; Suspected Pseudomonas aeruginosa, the culture is completed; Suspected Shigella, the culture is completed...

Including today's Vibrio cholerae, he has cultivated, isolated, and verified seven pathogenic bacteria, and with three more, he can complete the task!

Woo... But why hasn't penicillin been found yet...

Obviously, he has worked so hard. Every day, he spreads out a table of petri dishes, sprinkles soil, dust, and all kinds of things he can think of into the culture medium of Staphylococcus aureus, Mycobacterium tuberculosis, and various bacteria...

Why?Lady Luck, why wouldn't she favor him?

"Ahhhhhhh - the sky is going to be cold, if it drags on, the melons will be gone!"

Lucky for penicillin!The strongest culture medium!No matter how cold the weather is, there will be no more antibiotics. He wants to get this antibiotic out quickly, so there is even less hope!

(Another [-] words! Counting noon, it's another plus!)

　Human technological progress is really a relay climb.

　　Gram (Hans Christian Gram) created the Gram staining method, which is indeed very, very great, but also benefited from the gentian violet dye made by some chemist before him...

　　Ask for a subscription, ask for a monthly ticket, ask for a recommendation ticket, ask for a book review...

　　Help little Gretel who can't make penicillin!

　　
　
(End of this chapter)