Reshaping life starting from the Junior Class of HKUST

Chapter 154 Sanger Gene Sequencing Method

Wu Zhe flashed through the information he had just read, and there was some thought in his mind.He looked up and said:
"Well, I have some ideas, but there are still too few materials and data. I need Brother Zheng to help me get the data together."

Tang Meng's spirit couldn't help shaking, she knew that Wu Zhe was not a casual person, so she said quickly:
"Actually, you can speak directly. Brother Zheng is a nice person, and he still takes good care of us schoolmates."

Wu Zhe nodded: "Uh, I see that he is nice, otherwise I wouldn't really want to help him lose weight. I really like drawing pictures for people when I'm fine!"

"Then tell me, what do you think?" Tang Meng showed a curious look.Wu Zhe has only been here for one morning, and he has discovered something.She also wanted to hear Wu Zhe's thoughts. Scientific research is not afraid of making mistakes, but afraid of lack of direction.

Wu Zhe smiled and said: "It's easy to say, but it may not be realized, so why not? I think there are two aspects that can be started. One is to replace the current algorithm of statistical data, and start with mathematics to see if it can be optimized. The second It is to consider whether the previous sequencing method can be replaced."

Tang Meng nodded again and again after listening.He said to Wu Zhe: "Ah Zhe, the first of these two methods is still possible, but the second is very difficult. You should know the current sequencing standards. Both methods were developed more than 20 years ago. After so many years No one has ever come up with a new theory."

The current gene sequencing method still uses the chain termination method pioneered by Sanger and Coulson in the 70s or the chemical method (chain degradation) invented by Maxim and Gilbert.
The core principle of the Sanger method is: since the 4' and 2' of ddNTP (four fluorescently labeled A, C, G, T bases) do not contain hydroxyl groups, they cannot form phosphodiesters during DNA synthesis Therefore, it can be used to interrupt the DNA synthesis reaction. Add a certain proportion of radioactive isotope-labeled ddNTP (respectively: ddATP, ddCTP, ddGTP and ddTTP) to the four DNA synthesis reaction systems, and then use gel electrophoresis and After autoradiography, the DNA sequence of the molecule to be tested can be determined according to the position of the electrophoresis band.

This method is now the mainstream mapping method. The pyrosequencing method, ligase method, etc. that appeared later, their core methods all use the dNTP in Sanger that can interrupt the DNA synthesis reaction.Therefore, the current methods are basically inseparable from the Sanger method.

That's why Tang Meng said that it would be very difficult for Wu Zhe to create a new surveying and mapping method.

Wu Zhe smiled and said: "I just have such an idea. Let's start with the Sanger method and see if the data calculation method can be improved! Then I will test the operation of the Sanger method myself, so don't let people think that I am After entering the Institute of Genetics, I don’t even know how to get started.”

"That's simple. The trouble is in gene comparison and reading. Basically everyone in the institute knows how to operate the Sanger method. How about I teach you?" Tang Mengjiao looked at Wu Zhe with a smile. It is rare to meet Wu Zhe who can't .

"Why are you looking at me like that? I haven't done it before, of course I won't. You really think I know everything!" Wu Zhe looked at Tang Meng and couldn't help pinching her nose lightly.

Tang Meng had class in the afternoon, so she went back to class before class.Wu Zhe began to study the previous experimental data.Then, I put all the experimental data in the past two years into my head, and began to deduce the commonalities and differences of these data in my mind.He also picked up the draft paper and started to write and draw.

When Zheng Yizhong returned to the office, he looked at the draft paper that had already been laid out on a table.He asked Wu Zhe, "I just left for a few hours, Ah Zhe, what are you planning to do?"

Wu Zhe didn't raise his head and said: "Brother Zheng, you bought Chinese medicine? Let me see if I can optimize the algorithm. Then see if I can rewrite a calculation program?"

Zheng Yizhong immediately put down the things in his hands, walked to Wu Zhe and said, "Can it be done? If it is done, it will be a great contribution. The most lacking thing now is time."

Then he looked at the mathematical algorithms and models written by Wu Zhe.However, he watched loneliness.This paper is full of Latin symbols, each of which is still familiar, but connected together, it is almost the same as the Taoist talisman.

Seeing Zheng Yizhong's research, Wu Zhe couldn't help asking: "Brother, is there something wrong with me?"

As soon as Wu Zhe said the words, Zheng Yizhong shook his hand holding the paper, then cleared his throat and said, "Junior Brother, you are asking the right person. There is a big problem here."

Wu Zhe was also surprised when he heard this, he couldn't help but put down his pen, and looked up at Zheng Yizhong who was standing in front of him.The expression is also puzzled: "Where is the mistake? You are just a beginning. Normally, there will be no mistakes!"

"Hehehe, don't worry! I didn't say you were wrong, what I want to say is that you don't have many numbers when you do mathematical calculations, and the paper is full of letters?" Zheng Yizhong asked awkwardly.

Wu Zhe breathed a sigh of relief, then rolled his eyes at Zheng Yizhong.You can’t even understand it, and you still study it for a long time.Ignore him and continue to immerse yourself in calculations.

Are you good at math?Well, this time I was despised by a freshman student.But there is really no way.People who are good at mathematics are really awesome, and those who can't get up are really not good at it.After learning this mathematics, there are more symbols than numbers.

When Tang Meng got out of school, Wu Zhe was still modeling.This is much more difficult than in the Mathematical Modeling Contest for College Students.

Tang Meng's mathematics obviously passed the test.Flipping through Wu Zhe's calculation paper, I can roughly understand Wu Zhe's thinking.According to Wu Zhe's idea, the comparison time can be increased by one-third. Don't think that this data is not good.This is already a big improvement.

Knowing Wu Zhe's thinking, Tang Meng also joined in.The two teamed up, and the progress was much faster.

The sky also slowly darkened.

Zheng Yizhong said to the two: "Have you gone back? I can go first."

Wu Zhe replied with a smile: "Brother, let's go first, don't forget to take the traditional Chinese medicine, and see the effect in a few days. I still want you to help me?"

"That's nothing to say, as long as it's useful. I will definitely keep my promise." That's fine, I'll go first.

Wu Zhe and Tang Meng didn't arrive very late either, as long as Wu Zhe didn't want Tang Meng to accompany her hungry.Besides, the calculation data model has passed his mathematical calculation.Then there is programming.

"Mengmeng, let's go back too! It won't work in a while." Wu Zhe said to Tang Meng, who was still writing calculations.

"Okay! Wait for me to finish writing here." After Tang Meng finished writing, she handed the part she wrote to Wu Zhe.

After Wu Zhe picked it up, he looked at it quickly.Then praised: "It's really worthy of being able to suppress a person in high school, this level is not low!"

Tang Meng returned a look that you only know.

Wu Zhe smiled and told Tang Meng about Zheng Yizhong's reading of the formula on the draft paper in the afternoon.

"I thought Senior Brother Zheng was a master, and I was afraid that I might make a mistake, so I looked at him cautiously, and he said to me: There are too many symbols on it, and too few numbers. I almost froze."

When Tang Meng heard this, she also giggled.

(End of this chapter)