I really don't want to be a doctor

Chapter 287 Journey to Africa Jeffrey's Question
in the afternoon.

Jing Xiaoran was flipping through the government materials given by Lin Yitian in the laboratory.

This document records in detail the current status of the Ebola epidemic in the world, and also mentions some of the Chinese government's requirements for scientific research assistance.

Its purpose is mainly divided into two points. First, through clinical and scientific research assistance, it demonstrates the responsibility of China as a major country; second, while helping African countries, the government hopes that the scientific research team that went to support it will be able to respond to the Ebola epidemic. To make breakthroughs in this field, the biggest goal is to be able to develop related vaccines.

This time, the government recruited four scientific research teams across the country to bring together experts in the field of infectious diseases and severe diseases to provide scientific research assistance to Africa; they are the public scientific research teams led by Kyoto Medical College, West China Medical College, and Fudan Medical College, and A private enterprise scientific research team led by Huaxia Li's Pharmaceutical Factory.

If Shenzhou Laboratory chooses to go to Africa for support this time, it will belong to the team of Li's Pharmaceutical Factory.

"Xiao Ran, Jeffrey is here to look for you."

Jing Xiaoran was watching intently, Lin Yitian walked into the laboratory and said.

Previously, Jing Xiaoran and Jeffrey originally made an appointment at noon, but in order to accompany Weng Huijin, they changed the appointment time to the afternoon.

"Okay, where is he now?" Jing Xiaoran looked up at Lin Yitian, "I'll go find him right away."

"Jeffrey is in his 'Mesenchymal Stem Cell' laboratory." Lin Yitian said.

This time, Jeffrey mainly discussed with Jing Xiaoran about the mesenchymal stem cell experiment. He had doubts a long time ago, but at that time the "immune checkpoint inhibitor" project was in full swing, and Jing Xiaoran devoted himself to it. He's not good at getting in directly.

Now that the "Immune Checkpoint Inhibitor" project is on the right track, Jeffrey also began to learn from Jing Xiaoran.

When he came to Jeffrey's laboratory, he was looking down at a slide with a microscope.

Jeffrey's "Mesenchymal Stem Cell" laboratory is very large. The main experimental positions are filled with various test tubes and reagents, and there are several books on the table.

"Boss, you are here!"

Hearing the movement, Jeffrey immediately raised his head, and smiled when he saw Jing Xiaoran.

Jeffrey is used to calling the leader of the laboratory the boss, which is unique in the entire Shenzhou laboratory.

"Well, how is the experiment going?" Jing Xiaoran walked to Jeffrey's side.

"It's not going well." Jeffrey looked away from the microscope, "Boss, sit down, I'll find you a chair, let's sit and talk."

"Alright."

So Jeffrey brought another wooden chair, and the two sat side by side.

"Boss, this is it." Jeffrey began to talk, "I have a few questions."

"Tell me." Jing Xiaoran nodded and listened carefully.

Jeffrey took out a large stack of A4 paper from nowhere, and the paper was filled with various questions in English.

"First of all, what are the identification criteria and functions of mesenchymal stem cells cultured in vitro?"

"Then, as a kind of stem cells, what components are the microenvironment of mesenchymal stem cells composed of? How do these components regulate the proliferation and differentiation of mesenchymal stem cells?"

"Does the mesenchymal stem cells cultured in vitro obey the strict rules of asymmetric division? If yes, then why do the expanded cells still have multilineage differentiation ability? The mesenchymal stem cells expanded in vitro have Is it transplantable? How do the mesenchymal stem cells that enter the body and die?"

After Jeffrey asked these series of questions, Jing Xiaoran was stunned.

What are these?

In his previous life, he did not have much research on "mesenchymal stem cells" and knew little about it.He couldn't answer many of Jeffrey's questions.

But seeing Jeffrey looking at him full of curiosity, Jing Xiaoran couldn't bear to let him down, racking his brains to recall information about mesenchymal stem cells.

"Show me your experiment process." Jing Xiaoran said to Jeffrey.

Hearing this, Jeffrey immediately passed the A4 paper in his hand to Jing Xiaoran. Jing Xiaoran picked up the A4 paper and began to study, and at the same time told Jeffrey some "basic knowledge" from time to time.

"As far as I know, the growth of mesenchymal stem cells must meet the following conditions: ① Cell adherent growth. ② The positive rate of CD105, CD73 and CD90 in the cell phenotype must be greater than 95%, while CD45, CD34, CD14, CD19 and The positive rate of HLA-DR must be less than 2%. ③ Mesenchymal stem cells have the ability to differentiate into osteoblasts, adipocytes and chondrocytes..."

These "basic knowledge" have been verified in the previous life, but they have not been verified in this era. There are only sporadic articles published in this field, but they have not been recognized by mainstream experts.

Therefore, the so-called "basic knowledge" is also for the previous life.In this day and age, it is definitely an advanced research result.

As soon as Jeffrey heard Jing Xiaoran say a few words, he immediately found a stack of paper and pencils on the laboratory table, "Boss, please speak slowly, I need to record it."

Jing Xiaoran nodded, and said: "These are my current personal conjectures, and they are all relatively simple understanding. Regarding your remaining questions, there may be nothing I can do."

Jing Xiaoran's knowledge can only reach this level, and he doesn't understand other more profound knowledge.

"Wait." Jing Xiaoran suddenly saw the last question Jeffrey wrote on the paper - why can mesenchymal stem cells be expanded in vivo but not in vitro?
"About this question, I should definitely explain it well." Jing Xiaoran thought for a moment, "This may be related to the environment of cells inside and outside the body. Nutrients are simulated by these in vitro factors. More importantly, adult stem cells cannot be expanded in vitro, which is related to their inherent division mode, and the determinants of stem cell division are transcription factors such as OCT-4, SOX-2 and NANOG Activity related to..."

For example, in vivo experiments and in vitro experiments, many experiments in vitro were carried out smoothly, but in vivo experiments all ended in failure.

Although in vitro experiments, experimenters will simulate the internal environment, but the internal environment of the human body is too complex to simulate a [-]% internal internal environment.Therefore, when the in vitro test is transferred to the in vivo, the details will often change, leading to the failure of the whole!

(End of this chapter)