Prime Minister Bizen

Chapter 13 The successful preparation of penicillin

In fact, when Hachiro searched for information about Shikanosuke in his previous life, there were many things that did not conform to the logic of ordinary people, whether it was the magic-modified plot of games, novels, or the plots originally recorded in history.

Hachiro put forward an idea of ​​his own here, and maybe Lunosuke really surrendered.But this deer is too annoying. In the process of reviving the Nizi family, there are too many small and medium samurai in the Maori family who have formed a deadly feud with him.The death of Shikanosuke is likely to be the Warring States version of "Entering the White Tiger Hall with a Knife" organized by many Maori samurai, and was finally directly hacked to death by the samurai who rushed up.

Hachiro remembers reading a document saying that the first knife to Kanosuke was cut by Kawamura Shinzaemon from behind Kanosuke, and after Kanosuke fought with him, he was swarmed by the Maori warriors. Killed with a knife.

Does this plot resemble the plot in which each of the 12 suspects stabbed the kidnapper to death in "Murder on the Orient Express"?In addition, if Shikinosuke really wants to assassinate Mori Terumoto or Yoshikawa Yuanchun.Why not carry weapons?Why did you use the word 'scuffle' instead of words like fighting after being slashed by Kawamura?What about Shianosuke's own katana?
As I said earlier, the Maori family’s system is said to be the alliance system of the noble clan. The Maori family is only their ally, not their lord. In the face of public anger, even Takashi Kobayakawa, who took over Yamanaka Yukimori with 3000 stones, could only be able to admit that this has happened.

It is also worth pondering that the last person who collected Shikanosuke's corpse was actually the two Ashikaru who were captured along with him who did not even have a name.What about the other former Nizi family retainers in the mighty expedition last month?Why don't they show up?What about the other Nizi samurai who surrendered after being captured in the Last Moon City?Why didn't they come forward?So the political water behind the death of Shikanosuke is deep.

After lamenting Shikanosuke, Hachiro continued to bring a few people to prepare for the experiment of purifying penicillin.Hachiro and Kenjiro carefully picked out the penicillium on the orange peel and placed them in a petri dish filled with distilled water for 24 hours. After that, they put them through a funnel into a wooden barrel similar to the sake brewing barrel that the Akigami family ordered last time. Inside, of course, multiple layers of gauze need to be placed on the funnel to filter the strains.

Then pour rapeseed oil into Mutong, stir the liquid in the barrel fully and let it stand for 1-2 hours. Through these steps, the liquid in the barrel will be distributed into 3%. The top is the fat-soluble material dissolved in the oil. It is an insoluble substance, and the bottom is a water-soluble substance, and penicillin is precisely soluble in water.

We only need to open the opening of the drainage tank on the side of the bottom to dissolve the penicillin in the water. As for why the side opening is not the bottom opening, it is because the open bottom opening will cause vortices when the water flows out due to gravity. The liquid is passively stirred here.

Then I instructed Master Xuanzhu to bring the charcoal powder that was boiled and cooled in advance, pour the water containing penicillin extracted earlier into it, and stir well.Due to the active structure of charcoal, it will absorb all the penicillin.

After the stirring, Hachiro instructed Todo Takatora and Akigami Jiuji to take the multi-layer rack that was ordered last time, put the test tube on the top layer, and put the second layer of glass test tube on the next layer, because there is no rubber tube. , Hachiro can only choose to use silk cloth to drain water, but silk cloth can't keep water out, and lack of water-saving valve to control the water volume, which is also a limitation of this era.

Hachiro instructed Master Xuanzhu and Gao Hu to put these charcoal powders into custom-made leak-bottom glass test tubes, the weight of which should not exceed 1/5 of the test tube, and then slowly pour distilled water into it, so that the charcoal water flows into the second shelf. in the test tube.

As for why you don't do it yourself, because it's too short to reach.Then Master Xuanzhu and Gao Hu poured the filtered rice vinegar into the test tube. The principle is that penicillin is weakly acidic, and this step can further discharge the alkaline substances in the test tube.Finally, pour in the soda water.So that the penicillin dissolved in toner is dissolved out.Finally, the purified high-purity penicillin flows from the test tube into the lowermost porcelain bowl.

But this is just the end of the penicillin preparation process, do you really know your penicillin works?Then you need a drug test.The biggest difference between modern medicine and ancient medicine is that modern medicine has high reproducibility and high feasibility through comparison of a large number of control groups.Since penicillin is refined by the local method, without the detection methods of modern scientific instruments, it is impossible for Hachiro to judge whether the content of penicillin or other substances in the solution is effective with the naked eye.Only confirmatory tests can be performed.

Therefore, Hachiro will quickly cover the porcelain bowl and put different labels on it to understand the influence of the outside world on the penicillin in the porcelain bowl.Then, use tweezers to take small discs of one inch square, after infecting the penicillin in different bowls, and place them in the middle of another bowl containing Staphylococcus aureus. (The Staphylococcus aureus in these china bowls were generated by expanded replication from a uniform dish strain, controlling for independent variables.)
"Then we have to wait for 3~5 days. If a sterile area is formed around the wafer after 3~5 days, it will prove that the penicillin in the label is successfully configured. High school biology knowledge completed the penicillin configuration experiment in Japan in the 16th century. In the end, whether it was successful or not, the process can be completed is a very remarkable thing.Hachiro even felt that he was already floating on the clouds with excitement.

"Um, what is a sterile zone?" Kenjiro asked weakly. Hachiro looked at the boy who was a few years older than him, and thought of the Jin Guangsheng family who died because of him, and said weakly in his heart. After apologizing, he explained, "I told you earlier that penicillin is a super potent sterilant, and its existence is beneficial to the natural erosion surrounded by bacteria. It is evil.

The petri dish we see now, ahem, sorry, I mean as we see the meat paste in the porcelain bowl is full of evil spirits.If penicillin is effective, he will create a protected area around the disc, dispelling the evil in the area.The stronger the effect of the same medicine, the larger the dispelling range.Now let's wait for a miracle! "

After Hachiro dealt with the affairs in the laboratory, he left Qiu Shangjiu's house to guard the door and followed Master Xuanzhu and others out. Todo Takahu rarely returned to Shishan that day, but stayed at Guangzhen Temple and Hachiro to Xuanzhu together. The master studies the history of Sinology.

At noon 5 days later, everyone reunited in the laboratory to uncover the porcelain bowl lids of the experimental results one by one.No. 12 failed, No. [-] failed. Just when everyone was frustrated, everyone finally discovered the formation of a sterile zone in the No. [-] porcelain bowl, and found the existence of a sterile zone in the next few porcelain bowls.

"Skuyi~" Everyone was amazed at the efficacy of penicillin, and more importantly, they had never seen or heard of a medical faction like Hachiro.

Tengtang Gaohu held a stack of porcelain bowls and looked at the sterile area in the middle, which was shocked for a long time and could not be calmed down: "Is this evil? Is this something that can suppress evil? Master, you are really amazing, if you are not from the hall Son, you must be a great physician. You will surely heal thousands of people!"

Hachiro heard Todo Gao Hu's praise, and suddenly Chuuji coughed dryly from his upper body, stood on the chair and patted Gao Hu's shoulder and said, "Gao Hu, but a doctor may be able to treat one person, but as a father's son, my dream is to heal. This world!" Hearing that, everyone present looked at Hachiro with even more admiration.

Hachiro instructed everyone to store the corresponding number of penicillin porcelain bowls at low temperature, take out a small amount and put them in a test tube, and then pour the corresponding proportion into salt water and happily take the test tube back to Shishan.

Hachiro was still trying to trick Zhijia into drinking this unknown liquid. Unexpectedly, Zhijia heard that Hachiro said that it was a drug they formulated according to medical techniques to treat hunger, fullness and tuberculosis. Zhijia looked at the transparent liquid and looked at the tender show The family drank it like a child's joke.

In fact, since ancient times, the main culprit of gastric ulcer is Helicobacter pylori, which has a lot of residues in raw food, and Japan is a big country of raw food. The most famous raw sauce beef and sashimi are even more underdeveloped in this material. The main source of protein for society.But you must know that these foods have not been systematically sterilized, let alone bacteria, you can't see the parasite spores.

Therefore, I would like to remind all book lovers not to be obsessed with Japanese sushi and sashimi.Even if it has been systematically sterilized, it cannot be completely sterilized. In the first 10 years of the author, I often went to eat Japanese food because of my parents’ favor. I would buy a large plate of 150 sushi in college and eat it myself in the dormitory. I was young. He suffered from stomach ulcers and various digestive tract diseases.This is why the author is so clear about the preparation of penicillin soil method.Hey, it's all my own fault to say more.

Naojia lay down and rested after drinking the medicine, and Hachiro was like that.Every day, I deliver medicine to Zhijia after dinner at Zhijia. After delivering medicines for a week, Zhijia's complexion has improved significantly, and he can sit up by himself.

After Hachiro continued to deliver medicine for a week, the Nao family's qi and blood were normal and could participate in the assessment normally.Naoya, who saw his improvement, loved Hachiro even more.Recently, he has been holding Hachiro almost every day, and even played the game of riding a bamboo horse with Hachiro.Sharing a rare father and son time.

In March of the seventh year of Tianzheng (1579), several uninvited guests came to Shishan City.

Although Naojia has recovered, he may still choose to greet the two distinguished guests with a heavy body in front of his sickbed, perhaps out of weakness. 'Acting skills are familiar to Naoka, who has been lying in bed for half a year and performing all his life.

After the guest entered Zhijia's house, Zhijia started his Oscar-like acting.Hachiro, on the other hand, bowed his head to accompany him. He thought that it was just a trick for the scene, with no lines and no chance to show his face.

"I'm really sorry, Your Highness Zhuqian, for letting you see a guy like me who is about to die in such a place." With the help of Fu Zhifang, the Zhi family sat up and leaned on the chair to say this, but he was still left and right. Need someone to support him to prevent him from falling.Looking extremely weak.

"It doesn't matter, I came here with sincerity, and I also believe in the credibility of Izumi Shou."

'what?Do you trust my dad's credit?If my father had a reputation, how did my father-in-law, Nobuyoshi Yamanaka and Urakami Zongjing die?Is the name of the three wicked men of the Warring States period for nothing? ' Hachiro couldn't help but complain when he heard the words of the person who came.

"Lord Yuchai, I am so seriously ill that I don't dare to hope for the dream." Naojia said intermittently in a weak tone, "But before I close my eyes, I want to see the future of Yuxijia." On the samurai, he grabbed the hands of the incoming samurai, and looked at the incoming person with bright eyes. Due to the large range of movements, he frightened Fukunofang and Zhongjia who were on the side, and also brought Hachiro, who was sleepwalking, back to reality.

Hachiro looked up at the person, um~!Old acquaintance, isn't this Mr. Monkey!

The monkey looked at Nao's family and said, "I understand, since this is the case, I, Hideyoshi Hideyoshi, I will accompany the adults to Azuchi."

"Your illness is so serious, how can you still go out to catch the wind? What if there is an accident on the road." said Fu Zhifang, who was on the side.

At this time, the monkey looked in the direction of the speech, and the person who was speaking in the direction was a young and beautiful woman. Although she was nearly 30 years old, she was well-maintained. She didn't have the charm of a mature woman. She was a little embarrassed, but the samurai in the back seat reminded Hideyoshi, and Hideyoshi realized his gaffe.

Of course, Hideyoshi's gaffe was seen by everyone present, including Naoka Ukita, who was pretending to be sick, but it's not unfortunate that he was the actor of Ukita. This cuckold incident did not affect Naoka's performance at all.

He pretended to cough a few times and covered his mouth and nose with the handkerchief he had prepared in advance. When he took it away, there was already a patch of blood on the handkerchief.Seeing this scene, Zhongjia and Fuzhifang leaned up and surrounded Zhijia, "Brother (His Royal Highness), what's the matter with you, hurry up, pull up the screen door, don't let the wind outside me blow in!"

It turned out that for this meeting, Hideyoshi was completely at ease, and he specially opened all the doors of his palace to show that he had absolutely no intention of ambush.Now Zhijia coughed up blood, and Fu Zhifang, who didn't know the real situation, was worried about Zhijia's body and wanted to tell his attendants to close the door, but was interrupted by Zhijia's wave.

"Stop!" Naoya said in an extremely painful tone, "Your Highness Yuchai, you can see that my body will not be able to support it until that day. I am here to give you Hachiro and Afu. I hope you can In the future, I will take care of Yuxijia and Hachiro as a child. This is my last wish as a person who is about to step into Huangquan." After speaking, Naoka bowed down and bowed to the monkey. "please."

"I understand what you mean, Izumi Shou, don't worry, I will take good care of Hachiro and Afu, and I will never let them suffer in half. You can rest in peace in Shishan. I will take them to Azuchi." Hideyoshi After thinking for a moment, he agreed to Nao's request, but he heard a different taste in Hachiro's ears, 'You can rest assured, your son is like my son and nephew, and my wife is like my wife'?

"Thank you very much, then please." After the Nao family bowed, Hachiro followed the Nao family to salute Hideyoshi, and the scene ended in this way. The gift prepared by Naoya Ukita left Ishiyama and went to Himeji.Hachiro went with Master Xuanzhu, Kenjiro, and his family of Akigami.

Perhaps it was because of yesterday's scene that Hideyoshi had a sense of superiority as a daimyo who gave 50 shi (at this time, only Nagahama and Himeji were 20 shi). Three old acquaintances in the ranks.

(End of this chapter)