This doctor has a system

Chapter 324 305. New test kit that attracts much attention
  "Would you accept being a family member for free?" Li Youliang asked.

"Free? That must be acceptable." Director Shi looked at him as if he was mentally retarded, "We are not fools!"

Don't take advantage of the cheap bastards.

"Okay, I understand." Gao Feng stood up, "We are going to launch a project, and we can combine it with our department."

"What project?" Director Shi felt itchy, "Mycoplasma drug resistance gene detection?"

"Yes." Gao Feng didn't sell out, "I want to do a project around this outbreak of mycoplasma pneumonia, and drug resistance gene detection is an important part of it."

"This test is very expensive!" Director Shi reminded, "If you use this to apply for a project, you will definitely not be able to get it approved."

Mycoplasma resistance genes are rarely developed now. There are only two laboratories in Z state doing it. Labor is very cheap. After all, there are dividends there, but the detection reagents are very expensive.

Gao Feng analyzed the principle of detection, which is to use PCR to amplify genes and then detect specific gene fragments.

Logically speaking, this is not a difficult task, but I don’t know why it is so expensive.

"Is it easy to disclose your methods for detecting mycoplasma and its resistance genes?" Gao Feng asked.

Huang Guangxun of Guangsheng Testing Company hesitated for a moment, but then thought that this seemed to be no secret in the industry.

"The technology used is PCR gene amplification." He replied.

"Then why is the fee so expensive?" Gao Feng asked puzzledly, "Even for a single person to board the plane, the price should not exceed 1000 yuan."

"How is that possible?" Huang Guangxun felt like he had met an amateur. "The electrophoresis PCR method is very complicated to perform, and the reagents are so expensive. If you spend 1000 yuan, you will lose your pants!"

"Electrophoresis?" Gao Feng was stunned for a moment, "Isn't it fluorescent PCR?"

"What? Fluorescent PCR is more expensive, right?"

It turns out that these testing companies all use the electrophoresis PCR method. This method is very slow, but its advantage is that it is simple and easy to operate and has a good accuracy rate.

Fluorescent PCR has the advantages of high sensitivity, high sensitivity, and high specificity, and is very suitable for microbial detection and genotyping.

"But it is expensive." Huang Guangxun said, "This does not require a large number of reagents, but the testing equipment is very expensive."

"How many servings do you have to make to recoup the cost? Isn't this a joke?"

Mycoplasma drug resistance gene testing itself is relatively niche, with very few people doing it. Their company can't do dozens of tests a year, so spending millions to buy instruments is just ridiculous.

It would be great to have an electrophoresis testing machine. This is to increase the visibility of the testing center, otherwise the company would not buy a piece of equipment with such a low utilization rate.

Gao Feng became calm, and then a smile appeared on his face.

It turns out that the testing equipment is expensive, so that’s just right. He has plenty of money, and he won’t waste it. The company's laboratory also needs 1-2 fluorescent PCR detection instruments, so I bought it at this time, killing two birds with one stone!
  "Youliang, go buy it quickly."

"Okay, I'll go now." Li Youliang quickly contacted Zhao Xingye.

"It's not cheap." The latter smacked his lips as he looked at the quotation, "You want to buy two units at once?"

"Hurry up and sign. The boss asked me to buy it. Who dares to say no?" Li Youliang urged, "Let them send it over the day after tomorrow. It must be installed and debugged this week."

Zhao Xingye did not dare to neglect and immediately communicated with the company called Guanglan Medical Equipment. The other party was an old medical equipment agent. The company itself did not produce any medical equipment and instruments. They were a resource integrator.

To put it bluntly, he is just a second-rate dealer, but he is indeed capable and can find you various instruments and equipment in stock.

The asking price is just a bit high.

But is this a problem? For Fengxin Technology, it is obviously not the case.

This is the big boss's order, and Zhao Xingye is very active this time.

"Their test kit is not good." Gao Feng frowned, "It's okay that the price is high, but the accuracy is not that good."

But after hearing what he said, the engineer debugging the equipment was not happy.

"This is the most advanced fluorescent PCR detection kit in the world. Even the American devils use this!"

"What the American devils use may not be the best." Li Youliang was a little unhappy when he saw him like this. Don't you know that customers are God?

Can’t God just say a few words when he sees the product and is not satisfied with it? We paid for it!

"No, I mean this is currently the most advanced." The engineer did not dare to go too far when facing Party A. "The accuracy and sensitivity of this test are unique in the country."

"Several of our laboratories with national prefixes use this model, and it can definitely meet your experimental requirements."

"Yeah." Gao Feng nodded. He had no intention of saying anything to this unknown guy. The other person's rank was too low to understand his thoughts.

Gao Feng didn't have any objections to him. Domestic manufacturers really couldn't make this kind of high-end instrument. Even if they could, the quality was not good enough. Not only did the price have no advantage, but the quality control was poor and it was easy to break.

But what Gao Feng doesn’t know is that in another 10 years, domestic medical equipment production and manufacturing technology will usher in a blowout development, and domestic production will be better than imports. It will not be a foolish dream.

But it's obviously not possible yet.

Gao Feng was also very satisfied with the testing equipment. What he found almost interesting was the kit used for testing.

The principle of PCR method is very simple.

The polymerase chain reaction is a molecular biology technique used to amplify a small known DNA fragment in vitro1 2 3.

The basic principle is as follows:
  DNA will denature and unwind into single strands at a high temperature of 95°C.

When the temperature drops to about 60°C, specific primers bind to single-stranded DNA and pair according to the principle of complementary base pairing.

Then, at around 72°C, DNA polymerase begins to synthesize the corresponding complementary strand along the direction from phosphate to five-carbon sugar, and so on, in a cycle to achieve the purpose of DNA molecule amplification.

During the PCR process, DNA polymerase uses specific single-stranded DNA as a template and uses primers to start the synthesis process.

During replication, DNA gradually unwinds to form a series of Okazaki fragments. These fragments are connected under the action of DNA ligase to form a complete long DNA chain.

This technology was pioneered by Kary Mullis of Cetus Corporation in the United States in 1985 and can amplify trace amounts of target DNA fragments more than one million times.

Kary Mullis himself won the 1993 Nobel Prize in Chemistry.

"I can redesign a test kit that can improve sensitivity and accuracy." Gao Feng said while holding his chin. "The cost should also be greatly reduced."

The engineer on the side curled his lips when he heard this. He had installed and debugged equipment so many times, and this was the first time he had seen such an outrageous thing.

Who is this person? If it was illegal to brag, he would have been arrested long ago, he thought to himself.

"Don't leave for the next two days. We may need your help then." Gao Feng said to him.

"Boss, I'm very busy. If it doesn't work, we can communicate on the phone then." The engineer refused. He was telling the truth. The company has assigned many tasks. According to the plan, he will take the high-speed rail to Jiangsu and Zhejiang tonight. .

There is so much work everywhere that I can’t keep busy.

"If I ask you to stay for two days, just stay here for two days." Li Youliang said dissatisfied, "This is not a discussion with you."

"Boss, please go ahead and let me communicate with him." He said to Gao Feng. "No, my job is just to ensure the normal operation of the equipment." The engineer argued rationally, "I have no obligation to cooperate with you in doing other things."

"I left my words behind today. I have to leave at night, and the tickets are all booked!"

"I'll give you money." Li Youliang said, "You make a price."

"It's not about money. We've made an appointment with them," the engineer explained. "Two of the clients are from universities, and they're also in a hurry."

"Besides, do you think I'm the kind of person who is blind to money?"

"I have professional ethics, okay?"

After he finished speaking, he packed up his things and prepared to leave.

"Two thousand." Li Youliang said.

"Please don't insult me, okay?" The engineer looked unhappy, "I told you, I'm not the kind of person who only cares about money!"

"Five thousand."

"No, listen to me"

"1, 1 a day." Li Youliang said.

"Mr. Li, you are embarrassing me so much."

"2 a day." Li Youliang
  "Mr. Li, you"

"One day 3" Li Youliang was interrupted before he finished speaking.
  "That's enough, that's enough!" The engineer held his hand tightly and said, "2 a day is enough. If it's more, I won't feel at ease."

"Mr. Li, you are really like a dragon or a phoenix among people, and you are a good judge of people!"

"What should I say? Is he willing to stay a few more days?" Gao Feng asked, "Actually, if you are in a hurry, one day is enough. It's not a big problem if I stay overnight."

"There's no need to stay up all night." Li Youliang said quickly, "You have to pay attention to your health."

I've been counting on you all my life, he thought to himself, and we can't stay up all night.

"After I had an in-depth communication with him and was moved by affection and understanding, he expressed his willingness to stay and assist us." Li Youliang said, "It doesn't matter how long it takes."

"Then he's quite reasonable." Gao Feng expressed his appreciation for this behavior.

Anyone who gives so much money should be reasonable. Li Youliang suddenly thought of a classic film and television scene.

In Justice League, The Flash asked Batman: What is your superpower?
  The latter told him: I'm rich. He really has money.

Gao Feng is much more awesome than Batman to some extent, he has a system.

"System, I want to spend 100 skill points to redeem mycoplasma detection and drug resistance gene detection kits."

"It has been redeemed for you. You need to follow the specific experimental process in the space to fully master its principles and reproduce the kit." The system prompted.

This is not difficult at all. Gao Feng found that compared with the old model, the principle of the new test kit has not changed fundamentally.

Only changes were made in the packaging and selection of fluorescent probes.

The new fluorescent probe binds to the target area more easily and fuses with the DNA, resulting in the emission of a fluorescent signal of a specific wavelength, which can be captured and analyzed by the instrument.

At around 9 o'clock in the evening the next day, Gao Feng completed the analysis and reproduction of the new test kit.

After thinking for a while, he called Hu Jiaran, who was working overtime in the laboratory.

"I'll go to your place later."

Hu Jiaran hung up the phone, thought about it, and quickly called Li Youliang to tell him that he was on a call, so he asked everyone to get off work.

"Stop it. Now hurry up and get off work. No one is allowed in the experiment for half an hour!"

"I haven't finished it yet." Wang Liang said, "We have reached this point, and the results will be available in two hours."

"The boss will be here soon. What will he think if he sees you working overtime?" Hu Jiaran motioned for him to get out of here. "We'll do it tomorrow. Don't miss this time."

After everyone was gone, Hu Jiaran breathed a sigh of relief. Why is the boss here?

It's definitely not based on the progress of the test. He reported it on the phone yesterday.

It seems like nothing is appropriate at this point, right?

About half an hour later, Gao Feng arrived with Li Youliang.

"Let's go to the laboratory. I have a test kit that needs to be made."

"Huh?" Hu Jiaran was dumbfounded.

"Ah what?" Li Youliang wondered, "Let's go."

I just drove everyone away, and now the lab door is locked. Hu Jiaran was speechless for a while.

"Is everyone off work?" Gao Feng was stunned for a moment when he arrived at the door, and then realized that he had indeed come at the wrong time.

"Then wait until tomorrow."

"No, they just left. I'll call them back." Hu Jiaran said quickly, "Anyway, they live not far away."

"Just call 3-5 people. It's a very simple gadget and it takes about 2 hours." Gao Feng said.

Most people don't like to work overtime, but if they can work overtime with a generous boss, most people will not refuse.

To be honest, you may not have this opportunity. The five people who finally came were Wang Liang, Yue Penghui, Gong Fanshun, Lu Yingtao, and Ma Zhansheng.

Ma Zhansheng is a member of the ECMO development team Mu Yongde. He has a very flexible mind and currently has a place in the entire team.

"Have you made any progress in the research and development of oxygenators recently?" Gao Feng still remembers him. In fact, with his current mental strength that is beyond ordinary people, it is difficult to forget people and things that he wants to remember.

"Thanks to the ideas you provided, we have made great progress now." Ma Zhansheng was very excited that the big boss recognized him, and his voice trembled a little when he answered.

Gao Feng nodded.

"The reason why I called everyone here so late today is"

"Hey, it's easy." Wang Liang was the first to express his opinion, "I can't do the previous steps, but leave it to me to encapsulate it, and I guarantee that it will be beautiful."

Gong Fanshun and Lu Yingtao were the main ones, responsible for the implantation of fluorescent probes, while Ma Zhansheng and Yue Penghui helped on the side.

"It'll take 2 hours." Li Youliang said softly, "How about we go back first and let them send it back early tomorrow morning when it's ready."

Staying up late is the worst thing for the body. He didn't recommend Gao Feng to come so late in the first place.

(End of this chapter)