This doctor has a system

Chapter 398 463. My idol is awesome!
"Famous in Jinghua: Although you have gained considerable popularity in the medical field, it is far from enough in the eyes of the real big names. It is not because you are not good enough, but because they think you are young and may not be good enough. Please participate as soon as possible Ran Manyun came in for treatment and worked hard to alleviate her condition." Gao Feng was about to leave, but the system jumped out at this time.

Aren't you messing with me? Gao Feng was speechless inside.

He specializes in respiratory medicine and is not good at blood tumors. It would be very abrupt to jump out at this time.

After careful consideration, he still didn't dare to say a word.

"Teacher, do you think there is no other way?" Gao Feng asked Academician Bao on the way back.

"I'm afraid there is no good treatment method at present." Academician Bao replied. If there were, the patient's family situation would have used it long ago.

Some diseases are so equal that everyone is treated equally, even if you are a billionaire or in a high-ranking position.

"I'm quite interested in this refractory diffuse large B-cell lymphoma." Gao Feng gave the teacher a vaccination first, "I'll look up the information on this when I get back."

"Okay." Academician Bao said, it is a good thing that young people are willing to learn.

After returning home, Gao Feng first called Li Xinyao and chatted for a while, and then directly entered the system space.

Academician Bao thinks so. The patient's general condition is inherently bad. If you don't mess with her, she will die. What if you do something and the family blames you?
He has a lot of energy. Even if he is an academician, Gao Feng might also be punished.

Finally, the expanded CAR-T cells are infused back into the patient.

"Who developed Noxinaxin Sodium Injection?" What surprised Academician Bao was that the bedside doctor actually knew about Gao Feng.

Then genetic engineering technology is used for embedding modification, and the T cells immediately transform into tall CAR-T cells. It is no longer an ordinary T cell, it is a T cell with GPS navigation that can accurately find cancer cells, launch a suicide attack, and die with them!

Although it certainly has the disadvantage of being expensive, it is very effective. The treatment plan is described as follows: it can "clear" cancer cells within two months, which is better than traditional radiotherapy, chemotherapy and ordinary targeted therapy. All good.

"Yes, yes." Another family member also said, "Xiaoyun is just waiting to die here. If you really have any means, get her killed as soon as possible."

So the question is, how to tell the patient? You know, the first step is to isolate immune T cells from the patient.

Academician Bao stopped talking about improving this. To be honest, Nosinaxin sodium injection for spinal muscular atrophy is famous in the industry.

It is not a simple medicine, but a living CAR-T cell customized for patients.

"Are you really sure about this?" Academician Bao hesitated.

No wonder such a task is issued. System, I wrongly blamed you.

This means that the technical threshold of the drug is extremely high, it is specially tailored for patients, and its scope of application is small. One drug can only be applied to one type of cancer.

The CAR-T cells that are infused back into the patient's body are Akilencel injection.

"This is not our specialty, but I have done it."

"Oh my god, he came here yesterday too!"

"It's you!" Gao Feng immediately cashed in 300 skill points.

Hearing these words reassured the doctor, and Academician Bao suddenly felt relieved by more than half of his stress.

To culture and expand CAR-T cells in large quantities in vitro, a patient generally requires billions or even tens of billions of CAR-T cells (the larger the body, the more cells are needed).

"Academician Bao, do you have any new ideas?" the doctor in charge also asked expectantly.

"80%." Gao Feng said.

Gao Feng thought for a moment and decided to go find the teacher. Academician Bao didn't say a word for a long time after listening to his words.

As a developer, Gao Feng was very popular during that time. This was the first orphan drug in China, which was of great significance.

For no reason, even an ordinary person would not be able to make you suffer, not to mention the identity of the patient is very unusual.

"What age it is, if something goes wrong we can't blame the doctor who treats the disease." Ran Manyun's mother said, "Leave aside the feudal society, the emperor still doesn't kill the imperial doctor!"

In fact, Ran Manyun's family communicates very well.

"your student?"

"Then let me communicate with the family first." Academician Bao thought for a while and said, "Listen to what they have to say."

"Of course I know. He is the youngest Yangtze River scholar. He also made the first imitation of bevacizumab injection. It seems that there is also a digestive capsule vibration system, which can naturally be used in series." The eyes of the doctor in charge were filled with admiration. , "She seems to be 5 years younger than me!"

The specific implementation steps of this therapy are: first, isolate and extract immune T cells from cancer patients.

It took him a few days to learn and write the entire process, and then he would officially enter the experiment.

In fact, what he wants to say is, is your brain kicked by a donkey?

"Why didn't anyone tell me?"

A drug is mentioned in the treatment plan: Aquilenza Injection, as an autologous immune cell injection, genetically modified autologous targeting human CD19 chimeric antigen receptor T cells by a retroviral vector carrying the CD19 CAR gene preparation.

"It's not me, it's my students."

To his surprise, there were indeed treatment options for refractory diffuse large B-cell lymphoma in the system.

"I'm relatively confident." Gao Feng knew that the teacher was doing it entirely for his own good. "Don't you know the Nosinaxin Sodium Injection that I led the research and development of?"

"Yes, that's the one I brought with me yesterday, Gao Feng." Academician Bao said to him, "He"

"Your brain," Academician Bao said, "What are you thinking?"

Chimeric antigen receptor T cell immunotherapy is also very novel. Gao Feng feels that this is a very promising treatment method for malignant tumors, not just limited to lymphoma.

"We won't blame you even if we die!"

"This is not our job, there is no need to rush forward." He said to Gao Feng, "My status is very special. If anything happens, it will be blamed on you. How unjust you are."

Academician Bao.

"What did Professor Gao think about it?" the doctor in charge asked, "He must have a particularly genius idea."

This is like meeting a fan of his disciple, Academician Bao thought to himself.

"He wanted to develop a drug specifically for patients. This is the principle."

"Oh my God!" Before Academician Bao finished speaking, the doctor in charge shouted, "Awesome!"

"My idol is awesome!"

"Developing drugs directly for patients will not frighten those old men and women academicians who are too arrogant!"

The old man, Academician Bao, looked at him deeply. "I didn't talk about you." The doctor in charge said quickly.

The family members also quickly learned the information about Gao Feng from the doctor in charge of the bed. They verified it from the side and quickly built up a lot of confidence.

"Xiaoyun, maybe there is hope." Ran Manyun's mother had a smile on her face, "You must hold on."

Ran Manyun disagreed in her heart, but still nodded. She didn't want to make her mother too sad in the last stage of her life, at least not while she was alive.

"I need to collect your blood samples." Gao Feng said to Ran Manyun, "The purpose is to isolate immune T cells."

Seeing such a handsome doctor, the latter's eyes lit up.

"You look a lot like my ex-boyfriend," she said.

"Nonsense, your ex-boyfriend is not that handsome." Ran Manyun's mother said matter-of-factly, "This guy is much more handsome than the guy named Wan."

The praise from patients and family members put Gao Feng in a good mood, and he decided to work harder to get a satisfactory result for the two discerning ladies.

Hu Jiaran received the call soon.

"Take someone to the capital?"

"Yes." Gao Feng said, "You personally bring 50 people here. Wang Liang and Dr. Lu also want to come."

"As soon as possible!" After saying that, he hung up the phone.

Hu Jiaran immediately called Yue Penghui and asked him to call someone. The latter looked confused.

"I don't know what's going on." Hu Jiaran said to his confidant, "The boss just called me and didn't go into details. Let's go and prepare first."

Fortunately, there is not much work in the laboratory now, so smoking 50 people should not have much impact.

"There is a patient with lymphoma, but the treatment is not effective, and the boss is going to develop a drug specifically for her." Li Youliang said to Hu Jiaran, "Let's continue doing what you are doing."

"Then why come to the capital?" Hu Jiaran complained, "It may not be as convenient here as us."

"The patients don't want to come to us." Li Youliang said, "But in the early stage, we have to collect blood every day, so we can only let you come."

It’s quite stageable! Hu Jiaran thought to himself, but he just said this in his heart, knowing that the patient must have an unusual status.

After seeing Gao Feng, he naturally took the pamphlet handed over by the latter and frowned after reading it carefully.

The extraction of T cells in the early stage is very simple, and they will definitely be able to do it perfectly, but the step of producing CAR-T cells is very difficult, and they have not done anything similar yet.

Generally speaking, the topic was too high-end, and they were probably blinded.

"Don't be fooled by his name." Gao Feng said with a smile, "Genetic modification sounds fancy, but that's actually what it is."

As long as we understand the principle, I firmly believe that Hu Jiaran can do it with his hands-on skills.

"The principles are probably enough for me to ponder for a while." Hu Jiaran was not so optimistic. He had no knowledge of this at all, and it would be too troublesome to learn it from scratch.

"Let's do it this way. I'll tell you how to do it, and you just do it." Gao Feng thought for a moment and said. He asked Hu Jiaran mainly because of the latter's hands-on ability.

Thanks to the strong coordination ability of the patient's family, everything went smoothly.

The first step is to separate T cells from peripheral blood using centrifugation and other methods, and further wash them to remove red blood cells and other impurities.

Even low-level scientific research workers can do this step, so there is no pressure on the team.

In this step of genetic modification, you must first select a suitable retrovirus or other viral vector. The one that was highly selected was gamma-retrovirus. Don't ask why, it was just a whim.

"Then what method is used to transfer the foreign gene encoding the CAR protein sequence into T cells?" Dr. Gong asked. This step is obviously the biggest difficulty. He and several others have been thinking about it for a long time, but they still have no idea.

"Is it possible to use the ionization conduction method?" Zhao Kang said, "I participated in a project before, and they used this method, but the success rate was not very high."

In fact, the success rate was not low, but extremely low. The project ultimately aborted, and the funder screamed for compensation.

"Use electroporation." Hu Jiaran said, "Let's set the voltage to."

"Successful!" Dr. Gong shouted. He was a little excited, but the rest was at a loss.

What’s the hard part about agreeing? That's it?
"Let me take a look!" Hu Jiaran immediately pushed him aside.

"Quick! Hurry up and verify whether the current T cells can express CAR!"

The verification results are very satisfactory, which means that the research and development process has taken a big step forward.

Next comes the activation and expansion steps. After adding appropriate costimulatory molecules such as CD3/CD28, the immunomagnetic bead system is used to stimulate T cells and induce massive proliferation of T cells through cells expressing costimulatory ligands.

"But we don't know this immune magnetic bead system." Hu Jiaran ran to find Gao Feng.

The principle of immunomagnetic beads is to use the core-shell synthesis method to synthesize composite materials containing superparamagnetic substances covered with polymer surfaces, which have good stability and can be labeled later.

The functional groups on the surface of these substances, such as amino groups, carboxyl groups, and sulfhydryl groups, are used for covalent or non-covalent coupling of antibodies, which can be used to bind the corresponding antigens or antibodies, so that they can move directionally under the attraction of an external magnetic field to achieve separation. , for the purpose of detecting and purifying genes, proteins, cells, microorganisms, etc.

It sounds simple, but it is very difficult to do. There are not many talents in this field in China.

"It's not that difficult. I'll tell you the steps and you do it." Gao Feng said to Hu Jiaran, who was speechless.

"Brother Feng, I only have a bachelor's degree." He reminded kindly.

"This is because practice makes perfect." Gao Feng explained, "Even if you have a doctorate, you have to be familiar with it step by step in the early stages."

He has been working hard in space for a long time and has only succeeded once, but he still has an inexplicable confidence in Hu Jiaran.

This kid is very handy!
"First, we need to prepare the cell suspension, tissue-split red-cell count-Nave cells-resuspension."

Hu Jiaran frowned.

"Secondly we need to enter the magnetic bead incubation process, magnetic bead components - pipetting - pipetting - use of magnetic poles - collection."

Hu Jiaran's face turned pale.

“Then comes the flow analysis process, clone number selection—death or death—gating logic.”

Hu Jiaran's forehead was covered with sweat, and he felt nauseated.

"What's wrong, I'm allergic to all this lofty knowledge!" he thought to himself.

(End of this chapter)