They’re all reborn, what’s wrong with being a little more almighty?

Chapter 144 A little test
In the biology department laboratory, Zhang Wei has already put on a white coat, hood, mask and goggles.

Several graduate students gathered around and looked at Zhang Wei curiously.

"Who is the classmate that Director Meng brought? He looks quite unfamiliar! He shouldn't be from our department, right?"

"Of course it's not from our department. He is Zhang Wei!"

"Which Zhang Wei? I know several people named Zhang Wei."

"It's Zhang Wei who invented graphene. In April, several waves of leaders came to inspect, all because of him. There are photos on the school's official website!"

"It turns out it's him! What's he doing in our biology department? As far as I know, graphene should have nothing to do with our biology department!"

"Looking at this posture, it looks like we are going to do an experiment, right? Isn't graphene made of carbon? I haven't heard that it is a biological material!"

When everyone around him was muttering, Zhang Wei was already standing in front of a flow cytometer, and then he said, "I'll just do a flow cytometry!"

"You want to do flow cytometry? Are you sure? Although this experimental operation is basic, it cannot be done without training." Meng Heming said.

"More than enough." After Zhang Wei took the sample, he immediately started processing it.

However, the preparation of single cell suspension is only the most basic step of flow cytometry. The real difficulty is the subsequent data analysis and analysis.

At this time, Zhang Wei had turned on the flow cytometer and preheated and calibrated the system.

Meng Heming, who was watching from the side, nodded slightly. Zhang Wei's operation seemed to be decent.

Zhang Wei looked at a graduate student next to him and said, "Prepare a sample for me. I want to prepare a single-cell suspension!"

However, for Zhang Wei, these operations are not difficult. After all, flow cytometry is widely used in testing, such as the inspection and quarantine of poultry and livestock.

"Then let me show you how to use a microscope? Or observe individual oral epithelial cells?" Zhang Wei asked with a smile.

As Zhang Wei spoke, he started the next step, introducing the processed samples into the flow chamber through the flow cytometer's sampling system.

"I just demonstrated the experimental steps, the laser calibration of the flow cytometer, the gain and compensation of the photoelectric tube, and I just set it up casually."

"Give me phosphate buffer!"

"Give me the enzyme digestive juice!"

It didn't take long for Zhang Wei to complete the entire set of flow cytometry operations.

The graduate student looked at Meng Heming, and Meng Heming said, "Follow what he said!"

"Where's the cell counter!"

Zhang Wei continued: "Director Meng, if I really performed this, I wouldn't be able to pass your level! Okay, let's do the flow cytometry!"

Flow cytometry is a technique for rapid detection and analysis of single cells in a fluid stream. This is an efficient experimental method, but its operation is complex and requires professional training and experience to accurately set experimental parameters and interpret data.

After measuring with a cell counter, Zhang Wei began to adjust the cell density and quickly reached the requirements of the experiment.

The next data parsing and analysis is the main difficulty of this operation, because the data generated by flow cytometry is very large and complex. During data analysis, it is necessary to adjust the gate and threshold to extract data from numerous cell populations. Useful cell subpopulations are screened out for analysis, so that the required experimental data can be obtained.

The graduate student immediately took a sample and said, "This is a mouse lymph node sample. Are these enough? There are more!"

"Give me saline!"

Meng Heming's face darkened, what do you mean? This is the laboratory of the Biology Department of Tsinghua University. Do you think this is a junior high school biology class?

"Very skilled! Like a seasoned expert, he has only a few years of practice to achieve this level!" Meng Heming looked at Zhang Wei in surprise again.

"The operational proficiency of many of my graduate students may not be as good as his. Where did he, a freshman, learn this kind of operation?

By the way, he shouldn't have been to the biology department laboratory, right? Who taught him these operations and the use of equipment! "

Zhang Wei's ability to operate physical experiments was average, but Zhang Wei was definitely good at operating biological experiments. After all, this was what he relied on for food in his previous life.

How to carry the rice bowl, only if you are familiar with it!

The graduate students watching around also had surprised expressions at this time.

"His flow cytometry operation feels smoother than mine!"

"Studying graphene is within the scope of physics, right? Do physical experiments require flow cytometry?"

"What are you thinking about! How can flow cytometry be used in physical experiments!"

"Then why does he know how to operate flow cytometry? And he is quite proficient! If he didn't have more than three years of experimental skills, he wouldn't be able to do this!"

At this time, Zhang Wei had already cleaned up the test bench and came to Meng Heming's side.

"Director Meng, am I good enough to come to the laboratory?" Zhang Wei asked with a smile.

The shock on Meng Heming's face did not fade at all. He could only smile to cover up his embarrassment.

"I have finally seen that there are real geniuses in this world!" Meng Heming thought to himself.

Zhang Weize continued: "Director Meng, I will start project research later, and you can pair me with two more graduate students!" "No problem, I don't have anything else here, just graduate students! I'll give you three!" Meng Heming Said without hesitation.

……

After showing off a wave of experimental operations in the biology department, Zhang Wei returned to Principal Gu.

"Have you reached an agreement with Lao Mengdu?" Principal Gu asked.

"It's settled, he will match me with three graduate students!" Zhang Wei replied.

"It is the first time for a college student to be paired with three graduate students to conduct experiments at Tsinghua University." President Gu paused and continued: "Since you have decided, then I will follow your project. Reported to superiors!”

"Thank you, principal, but when will this scientific research funding be available?" Zhang Wei asked.

"Are you anxious to do the experiment?" Principal Gu asked with a smile.

"I can't wait to start the experiment tomorrow!"

"Not that soon. Starting next Monday, it's possible!" Principal Gu replied.

"The funds will be approved next Monday? So soon? Didn't you say before that the fastest would be around New Year's Day?"

"How can funding be so fast! There is still some floating scientific research funds in the school. If you are in a hurry to do experiments, I will ask the finance department to grant you part first and let you start the experiments first. When the funds arrive, you can Pay back the money!"

Zhang Wei nodded: "Next Monday is not bad either. If I'm lucky and the experiment progresses quickly, I might have results before the funds come in at the end of the year!"

Principal Gu said earnestly: "Doing scientific research is not as simple as you say! Science must respect objective facts and must be done step by step. Don't be impatient. You can't eat hot tofu if you are impatient. When doing scientific research, you still have to do it. Calm down and take it one step at a time.”

Zhang Wei thanked him again and said goodbye.

Looking at Zhang Wei's leaving figure, a faint smile appeared on Principal Gu's face.

Principal Gu is actually not optimistic about the project proposed by Zhang Wei this time.

As Meng Heming said before, the project of inhibiting RNA recognition by Toll-like receptors has many research directions. It is a big project, and it is not easy to produce results.

Therefore, Principal Gu did not expect Zhang Wei to really produce much results.

Principal Gu even hoped that Zhang Wei's research would not go well and would eventually lead to nothing.

This was not because Principal Gu was evil-hearted, but because he wanted to take this opportunity to hone Zhang Wei's character.

Principal Gu felt that Zhang Wei, who had achieved such level of scientific research results at such a young age, would inevitably become restless and have his tail stretched to the sky.

For scientists, this is a big no-no!

Scientific research needs to follow the objective laws of the operation of things. If you want to obtain a scientific research result, you must proceed step by step. Opportunism is obviously not feasible.

Principal Gu hopes that after experiencing setbacks, Zhang Wei can learn lessons, truly concentrate on doing research, grow into a real scientist, and eventually become a leader in the domestic scientific field.

In Principal Gu's view, in order to allow a national leader of Zhang Wei's level to grow, it is not a big deal to spend the scientific research funds of one or two projects as tuition.

The top scientists cannot be measured by money. It is worthwhile to spend all the scientific research funds of Tsinghua University to cultivate one of the world's top scientists for the country.

If money could buy Einstein, I believe many countries would be willing to spend their treasury on it!

……

After leaving Principal Gu's office, Zhang Wei also smiled happily.

He said that he would be able to produce results before the funds came in at the end of the year. He was not lying. After all, he did not need to re-research. He only needed to do a new experiment according to the content of the paper, and then paste his experimental data into the paper. It’s ready for publication.

The title of the paper Zhang Wei copied this time is: Inhibition of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA.

This contains three levels of meaning. The first one is the inhibition of RNA recognition by Toll-like receptors, which is the topic Zhang Wei applied for.

The second is the impact of nucleoside modifications, which was studied within the framework of inhibition of RNA recognition by Toll-like receptors.

That is, through the study of RNA recognition inhibition by Toll-like receptors, the influence of nucleoside modifications was discovered.

The third level of the paper is the evolutionary origin of RNA.

The most important of these three levels is actually the impact of nucleoside modification. It was this part of the research that ultimately won the Nobel Prize in Physiology or Medicine.

When Zhang Wei was conducting experiments, the most important thing was to do experiments on nucleoside modification.

But when applying for scientific research projects, Zhang Wei did not dare to mention even half a word of nucleoside modification.

If it is stated clearly that nucleoside modification is required, it is equivalent to directly clarifying which path can be taken to win the Nobel Prize.

Zhang Wei hasn't done the experiment yet. He has pointed out the road first. What if someone runs up ahead of time?
Therefore, when applying for the project, Zhang Wei only mentioned research on the inhibition of RNA recognition by Toll-like receptors.

There are more than twenty kinds of Toll-like receptors. Who knows which one Zhang Wei wants to study? Who knows how far to go? Who knows how much can be researched in the end?

Just looking at this research topic, no one would have thought that it would result in a Nobel Prize!
(End of this chapter)