They’re all reborn, what’s wrong with being a little more almighty?

Chapter 199 I am not an old hapless person
In the Starbucks cafe, a young curly-haired man was looking across the street with an angry look.

Opposite him, sat a fat man. This man looked to be about 230 to 40 pounds, and his body was almost spherical. The obvious big belly was like a small hill, hanging abruptly on his waist. That round face, with a little childishness and addiction, is a typical portrayal of American otaku culture.

This fat man is Yang Dao, the creator of the DOTA map, and the Juan man opposite is Bing Frog, the creator of the DOTA map.

"Are you really not going to update DOTA? You have paid so much for DOTA, are you going to give up now?" Ice Frog asked.

"I have lost interest in making DOTA maps. The game Warcraft 3 has entered the saturation period of players, and there will be no development in the future. Why don't we try some new games with development potential!" Yang Dao said. road.

"Are you talking about World of Warcraft? I know that since the end of last year, you have been addicted to World of Warcraft, so you haven't updated the DOTA map." Ice Frog said.

"World of Warcraft is really interesting. You can take a look at the current game rankings. World of Warcraft has been ranked first for many months in a row. Everyone loves to play World of Warcraft. As for Warcraft, the number of players has dropped significantly.

We must admit that the most popular game right now is World of Warcraft. This is a revolutionary game. Its emergence has eclipsed other games. "When Yang Dao talked about World of Warcraft, he seemed excited.

This is not bragging by Yang Dao. The emergence of World of Warcraft has indeed made other games fall by the wayside. Many game studios have suddenly discovered that the projects they are developing are nothing compared to World of Warcraft!

Both the game graphics and game settings are several levels behind World of Warcraft. Their games are still being developed, but they are already lagging behind.

It is no exaggeration to say that the emergence of the game World of Warcraft has dealt a huge blow to the entire game industry. Countless game designers have also lost confidence because of this. They discovered that the gap between themselves and Blizzard is so big. Blizzard is a big mountain. , it is simply impossible to climb over.

Yang Dao probably feels like this now. Facing one of the best products from Peak Blizzard, he feels a little ashamed of himself. Coupled with his addiction to games, he can no longer pick up DOTA maps.

Ice Frog went on to say: "Did you know that during the few months you left, an officially certified version of the DOTA map appeared? This officially certified version not only added new heroes, but also fixed bugs and modified Lots of hero and item parameters!”

"It's a good thing if someone is willing to continue making DOTA maps. Then we can save our worries!" Yang Dao said carelessly.

"But this producer is not a member of our team!"

“If it’s not, then it’s not, and no one has stipulated that only we can make DOTA maps. The map editor is there, and anyone can make it!”

"If this continues, our previous efforts and achievements will be taken away by them."

"I don't care. Anyway, I made DOTA maps for fun. If you don't want to, then you can continue making DOTA maps!"

Yang Dao said, looking at his watch, and then said: "No more chatting, it's almost three o'clock. I made an appointment with my friend to download the dungeon together at three o'clock."

After Yang Dao finished speaking, he stood up and left, leaving Bing Frog sitting there alone with an annoyed expression.

Seeing Yang Dao's body weighing more than 200 pounds sitting in the cab, the whole car shook a few times, and there was a hint of unwillingness in Bing Frog's sharp eyes.

"I do what I do. I did all the main work in the last version. I'll go back and update the map now!"

After returning to his residence, Ice Frog went to the computer and started working on the DOTA map. He worked until early in the morning before stopping.

Ice Frog stretched and then entered Blizzard's forum, intending to see what new popular posts there were.

A bold post title immediately came into Ice Frog's sight.

"DOTA official certified version 1.30 is officially released! It's that official certified version again, and there's a new version, and it's updated pretty fast."

Ice Frog subconsciously clicked on this title.

The first few parts of the post are about adjusting hero parameters, fixing some bugs, etc.

The next line of text is specifically marked in red: This version update introduces the messenger system, which provides players with a new game experience and strategic choices. The following is a detailed introduction and usage of the newly added messenger system.

What follows is the overview, functions, usage, etc. of the messenger.

Looking at this update, Ice Frog immediately fell into deep thought. At this time, he had a premonition of the strategic significance of the messenger.

"Download the map and go in and take a look!" Ice Frog decided to experience the function of the messenger himself.

Half an hour later, a feeling of despair arose in Ice Frog's heart.

What Ice Frog didn't expect was that just adding a messenger mechanism would increase the pace of the game by several levels.

DOTA is not a casual game, but a multiplayer battle game. The pace of this kind of game must not be too slow. It is best to allow players to enter the battle phase from the beginning of the game, and to play PVP from beginning to end. A more exciting gaming experience.

The faster pace of the game means there will be more and more exciting PKs, which is exactly what players need.

Bingwa suddenly felt that the DOTA map designed by others and the DOTA map designed by himself seemed to be different from the same game. The appearance of the messenger directly gave the Ice Frog a dimensionality reduction blow.

"Should I also learn and add a courier to my DOTA map?"

This idea flashed through Ice Frog's mind, but then he rejected it.

Whatever others produce, you should follow suit. Isn’t this plagiarism?

Although there is no such thing as plagiarism in terms of game mechanics, it is common for game companies to add each other's excellent game mechanics.

But the player's eyes are sharp after all. Whether it was copied from other games, the player only needs to experience it once, and he will know clearly in his mind.

Ice Frog has always believed that the DOTA map he participated in the production of is the genuine DOTA, and the so-called official certified version is just an imitator. If he were to borrow from imitators, wouldn't he become an imitator himself?
He took a deep breath, quit the game, and continued reading the official forum posts.

Many players have already commented on the new design of the Messenger, and almost all of them are positive.

"The courier is a great design that can quickly transport items from the base to the hero, or from the hero back to the base. I no longer have to run back and forth between the base and the front line!"

"The messenger greatly improves the efficiency of item exchange in the game, allowing heroes to obtain the equipment and supplies they need faster. I give this setting a hundred points!"

"In the past, after a wave of team battles, I was always forced to go back for supplies, not only to recover, but also to update equipment. Now with this messenger, I can continue to fight on the front line."

"Have you noticed that the courier can also be used as a reconnaissance unit? You can use the courier as an early warning to prevent enemy sneak attacks, and it can also be used to detect the enemy's location. With this courier, we can use more tactics. "

Looking at these good reviews, Bingwa finally regained some of his mood, but fell to the bottom again.

At this time, he had realized that the setting of this messenger alone blocked his way to continue updating the DOTA map.

Ice Frog let out a long sigh: "Maybe Yang Dao is right, and I should give up making DOTA like him!"

……

In 2005, the most popular game in the world was World of Warcraft. The game also sucked a lot of players away from other games.

But not all players are used to the third brother screen. Many players who are accustomed to the second brother will feel dizzy after playing the third brother game for a long time. Especially the game graphics of World of Warcraft have a much higher layering than other games at the time, which is more likely to cause dizziness.

Moreover, the game World of Warcraft also has certain requirements for computer configuration. At that time, most computers could not run World of Warcraft.

If you follow the official minimum configuration, then sorry, you actually can’t play it. If there are few people in the scene, the performance of low configuration will be normal. If dozens of people download the dungeon and various skills are released, your computer screen will immediately turn into a slideshow.

If you really want to play smoothly, you have to use the official recommended configuration, and this recommended configuration is several levels higher than the minimum configuration.

For example, the official minimum configuration tells you that the integrated graphics card can meet basic gaming needs, but the official recommended configuration tells you that it is recommended to use the X700 series or 6600 series graphics card.

This is equivalent to going to a racing track and asking what kind of car you can race on for two laps. People tell you that a Jetta can run up and down the road, but we still recommend you drive a Porsche.

Between Jetta and Porsche, there are several Passats.

If you can't adapt to Sandi games, or your computer configuration can't support World of Warcraft, but you want to experience the fun of multiplayer battles, then DOTA is the best choice at that time.

From another perspective, it was World of Warcraft’s massive killings that gave DOTA the soil to rapidly develop and grow.

The emergence of World of Warcraft has eclipsed other online games. Many game manufacturers have postponed the release of new products, trying to avoid the edge of World of Warcraft.

There are also some manufacturers who simply resold their products. They know very well that their games are uncompetitive in the face of World of Warcraft.

The number of new games has decreased significantly, and the playability of old games is not as good as the confiscation time. This has also created a short window period in the game market.

Those players who cannot play Sandi games, or whose computer configuration cannot support World of Warcraft, have no new games to play, and they find it boring to play old games, so they can only be forced to experience other types of games.

MOBA games like DOTA just fill this part of the market and attract this part of the players.

DOTA itself also has many advantages.

Compared with traditional real-time strategy games, DOTA is easier to get started and simpler to operate. It does not require high APM and complicated operations, nor does it need to control an entire army.

Moreover, the battle between ten people is just right, not too much and not too little. It is easy to put together a game, and it will not be boring because of too few players.

Compared with the same ten-player shooting game, you don't need to have that high level of gun pressure and preview. You only need to click the mouse and press a few keyboards to complete the operation.

In addition, Warcraft 3 already has a player base of hundreds of millions. It is not an accident that DOTA quickly became popular due to word-of-mouth among players. …

The first complete experimental report has been sent to Zhang Wei.

Looking at the thick experimental report and a lot of experimental data, Zhang Wei had a headache, so he simply asked: "Sam, just tell me the results!"

The content of the report had already been memorized by Sam, so he directly introduced: "Based on the twenty-four genes you listed, we conducted two kinds of transfer experiments.

The first one is that we transferred these twenty-four genes into mouse fibroblasts respectively. The result was that all experimental subjects showed negative cell transformation, which means that there was no good news about successful induction. . "

"That also proves that genes alone cannot achieve the transformation effect!" Zhang Wei said.

"To be precise, these twenty-four genes alone cannot achieve the transformation effect." Sam added.

"Then let's talk about the second experiment!"

"In the second experiment, we transferred all twenty-four genes into mouse fibroblasts at the same time, and the result was positive cell transformation."

“What do transformed cells look like?”

"You may be disappointed. Although the transformation result is positive, the cells have not undergone obvious transformation! We put these cells into the stem cell culture system, and these cells cannot survive." Sam replied.

The stem cell culture system is designed to determine the success of induced cells. This system can only allow stem cells or cells with stem cell characteristics to survive, and is also a stem cell screening system.

Zhang Wei nodded calmly. This result was completely within his expectation. After all, except for those four genes, Zhang Wei found the other twenty genes to make up the number. If you just make up the numbers, it will be surprising if you succeed!

"A single gene cannot produce transformation, but twenty-four together can produce transformation. This at least shows that among these twenty-four candidate genes, there is at least one combination that has the ability to induce. So next, we Just need to find that inducement combination.”

"Sir Zhang, the problem is that we don't know how many genes this combination with inducible ability is composed of. It may be two genes, three genes, four genes, or even a dozen genes." A graduate student He spoke.

"Combinations of more than a dozen genes can be eliminated directly. One gene can express even several biological functions. If it only has the ability to induce, theoretically there is no need to use so many genes to form a combination." Sam replied immediately.

Zhang Wei immediately cast an approving look at Sam and thought to himself, Yuanfang, I haven’t even asked you what you think, but you already answered quickly!
The next second, Zhang Wei still asked: "Sam, what do you think?"

Sam immediately replied: "If this combination has only two genes and twenty-four genes, there will be a total of 276 different permutations and combinations. That is the best situation for us. Even if it is an experiment one by one, we will You only need to do 276 sets of experiments to find this combination.

But if this combination has three genes, there are 2024 different combinations. Although the number is somewhat large, it is not impossible to conduct experimental verification. With better luck, we may be able to find the results we want in the first 1000 sets of experiments. "

When Sam mentioned luck, everyone else couldn't help but roll their eyes at him. As an old unlucky man, you are the least qualified to mention luck, okay?

Sam was also slightly embarrassed, but continued: "If this combination has four genes, there are 10626 combinations. It would be more difficult for us to try them one by one through conventional experimental methods.

And if this combination has five genes, then there are 42504 combinations. It is impossible for us to conduct more than sets of experiments to find the results. "

"So we have to find a way to narrow down the scope of investigation. Give me the table of twenty-four genes." Zhang Wei said.

Immediately, a student rushed the form to Zhang Wei.

Zhang Wei looked at the table, pretending to be thoughtful, then drew a circle on one of the genes, then pretended to be thoughtful, drew a circle on the other gene, and said:
"I think these two genes are most likely to appear in the combination of induced functions."

Everyone looked around and saw that Zhang Wei circled the two genes Klf4 and c-Myc.

"Sir Zhang, why these two genes?"

Zhang Wei pretended to be a master without saying anything, and then looked directly at his Yuan Fang.

Sam had no choice but to say: "The Klf4 gene itself is involved in regulating important life processes such as cell proliferation, differentiation, and embryonic development. c-Myc is a gene that allows cells to proliferate indefinitely, acquire immortalization functions, and promote cell division. This The functions of both genes are reflected in stem cells."

"But c-Myc is an oncogene, right?" another asked.

"c-Myc is an oncogene, but the c-Myc gene plays a role in early events of the cell cycle, prompting cells in the resting phase to enter the dividing phase. For our first experiments, it is indeed the most likely to appear in the induction combination. Of them." Sam replied.

Zhang Wei showed a satisfied smile. He really found the right person to find Sam. If it were him, he wouldn't be able to win at all!

Zhang Wei then marked another eight genes with triangle numbers on the form.

"I think these eight genes are more likely. Including the previous Klf4 and c-Myc, there are ten genes in total. We will work on these ten genes first and exclude the other fourteen for the time being." Zhang Wei said.

"Then are we going to transfer all ten genes into the host cells according to the previous method?"

"Didn't Sam just say that one gene can express even several biological functions? If it only has the ability to induce, the theory does not need to use so many genes to form a combination. So we still have to combine these ten Genes are divided into several groups for experiments," Zhang Wei replied.

"That's the same question as before. We don't know how many genes there are in this combination." The postdoc said.

"So we need to confirm this first." Zhang Wei said and looked at Sam again.

This time, Sam didn't answer, but hesitated for a few seconds before saying, "I don't know."

Yuanfang can’t tell anymore!

Zhang Wei said: "Sam, I want to ask you, what is your lucky number?"

"It's 7." Sam said a lucky number for most Westerners.

"Obviously, this is not your lucky number, otherwise, you would not be so unlucky. Change it!"

"How about 5?"

"4? That's a good number! It sounds lucky."

"I said 5..."

"Okay Sam, your lucky number will be 4 from now on!"

Sam was stunned. There is a big difference between "four" and "five". No matter how bad your English is, you can't tell them apart!

Zhang Wei thought to himself and said: "We will divide each four genes into one group according to Sam's number 4!"

"What? Is it decided? Is it so hasty? By the way, before you made the decision, didn't you listen carefully to what was said? They said 5! I see that you like 4 clearly!" Everyone couldn't help but feel. complained.

Zhang Wei did not give everyone time to react, he continued: "We must have discussed before that the two genes Klf4 and c-Myc are most likely to be induced genes.

Therefore, in the next experiment, we will use these two genes as the basis, and then select two from the other eight genes to form a combination of four genes for the experiment. "

"Who has discussed this with you? Klf4 and c-Myc are obviously your own, right?" Everyone continued to complain.

"In this case, there are a total of 28 combinations. Let's divide them into four groups. Each group will do seven, and we will get the results soon. Sam, come and divide everyone into groups! I will divide the following 28 gene combinations ”

After Zhang Wei arranged his work, he ran away quickly.

……

Historically, researchers have used a gradual reduction in genes to determine inducing combinations. From 24, reduce it to 23, do experimental verification, then reduce it to 22, do experimental verification again, and so on.

If you find that deleting a gene cannot produce a positive result for cell transformation, then keep this gene and reduce the others.

After repeated screening and elimination, and a large number of experiments, the induction combination of the four genes was finally determined.

However, Zhang Wei did not intend to use this method, mainly because the elimination method was too time-consuming. Every time a gene is eliminated, a round of experiments must be done, from 24 to 20. Even if the genes can be eliminated successfully every time, twenty rounds of experiments will be done. Considering that there will be errors in the experiments, more than thirty rounds will be done. The experiment was normal.

If you exclude weekends and holidays, it would take more than half a year at the earliest.

For a medium-sized scientific research project, half a year is nothing, it can even be said to be short. But Zhang Wei can't afford to wait. He still wants to return to Tsinghua University next semester.

So this faster experimental solution can only be used.

Sam quickly divided the team members into four groups.

Zhang Wei also divided the 28 gene combinations into four parts, with seven in each group.

But when he was grouping, he specially assigned the ultimately successful combination of Oct3/4, Sox2, Klf4 and c-Myc to Sam.

Zhang Wei felt that it would be a pity if Sam’s excellent academic skills were wasted. So Zhang Wei planned to give him a chance to succeed, help him rebuild his confidence, and at the same time clean up his bad luck persona.

Another reason is that he has been using Sam as a tool, so he must give him some compensation.

Yuan Fang, who has been a Nian for so many years, is ordered around by Master Di every day. Why should she be allowed to be a Yan Shuang eagle for once?
Sam, it's time to tell the entire Princeton University that you're not an old bastard!

(End of this chapter)