Simultaneous Transmigration: Starting from the Main God Space

Step 3: Parallel Project - The Concept of Yin-Yang Escape and Clone.

Just as the two main cell lines began to proliferate steadily, Wu Xian's gaze fell on a refrigerator on the other side of the laboratory.

He walked over, opened the cabinet door, and an even colder aura wafted out. Inside was Shimura Danzo's pale and eerie arm, inlaid with ten three-tomoe Sharingan eyes.

He removed the arm and placed it on another workbench.

The ten Sharingan eyes, immersed in the culture medium, seemed to retain some kind of activity, their pupils unconsciously turning slightly, emanating a chilling and sinister aura.

"That's not enough."

Wu Xian analyzed the situation calmly.

"The power of the Sharingan originates from Yin Release, which represents spiritual energy. However, for this power to be stable and strong, it must be balanced by its opposite—Yang Release, which represents life energy."

He took out another, smaller but extremely well-protected container, which contained a few grams of active cell tissue from the First Hokage, Hashirama Senju.

The immense vitality contained within those cells almost bursts through the walls.

"Hashirama cells, as the ultimate manifestation of Yang Release, possess powerful vitality and physical energy, which are the best foundation for balancing and supporting the Yin Release power of the Sharingan. Just like when Madara Uchiha integrated Hashirama cells into his own body and finally awakened the Rinnegan... Although the paths are different, the principles are the same."

Furthermore, in order to enrich the source of "Yin Release" and increase the diversity of Sharingan genes to improve the possibility of evolving into a higher form, he also brought out the somatic cells and Sharingan cell samples he had collected, including those of Uchiha Itachi, Uchiha Shisui, and several other Uchiha clan members.

"This clone will no longer be limited to a single bloodline limit. Based on Hashirama's cells, it carries and integrates the power of multiple Sharingan, with the goal of creating a 'Yin-Yang Release clone' that possesses both ultimate vitality and ultimate eye power. It will represent the dual unity of 'creation' and 'destruction'."

Step 4: Precise operation and risk prediction.

Wu Xian took a deep breath and turned his attention back to the first and most challenging project – "Hamura's Ancestral Doppelganger".

The white eye cell line and the cadaver vein cell line have been initially stabilized.

He needs to start experimenting with cell fusion.

He chose a combination of electrofusion and virus-mediated fusion.

Under micromanipulation, he precisely juxtaposed a white eye cell and a bone marrow stem cell from a cadaveric vein, and applied a precisely controlled short-duration high-voltage pulse to temporarily create reversible perforations in the cell membranes of both cells, promoting cytoplasmic communication.

At the same time, a novel virus that has been highly attenuated and modified to retain only membrane fusion function is added as a "bridge" to further improve fusion efficiency.

The fusion process was monitored in real time under a high-powered microscope.

Most attempts failed, with cells rupturing or undergoing apoptosis under severe stress.

Occasionally, a very small number of cells may successfully fuse to form a "heterokinetic body" containing binucleates (or heterologous chromatin).

These precious heterokaryocytes were immediately isolated and carefully nurtured in a culture medium rich in fusion promoters and special nutrients.

The next step will be the more crucial "nuclear transfer" or "somatic cell cloning".

He plans to extract the nuclei from these successfully fused heteronuclear bodies, or the "hybrid cells" that may be formed in the future, and transplant them into the "characteristic recipient cytoplasm" that has been enucleated and provided by himself.

Through microcurrent or chemical stimulation, this reconstructed cell is induced to begin dividing and developing, eventually forming a complete embryonic stem cell line, which is then cultivated into a complete individual—his "Hamura Atavism Doppelganger".

The same cloning process will also be carried out simultaneously on the "Yin Yang Escape Clone" project.

He used Hashirama cells as cytoplasm donors (providing a powerful Yang Release life background) and then attempted to transplant cell nuclei (or processed chromatin) containing various Sharingan genetic information into them.

The Yin-Yang Release clones require even greater caution. Hashirama's cells are extremely corrosive; direct fusion could easily cause the host cells to be devoured and turned to wood by their powerful vitality.

He must first rigorously screen and weaken the intercolumnar cells, possibly by limiting their proliferation generations or temporarily suppressing their excessive activity with specific enzyme inhibitors, before attempting nuclear transplantation with his own adapted cells that carry the genetic information of the Sharingan cells.

Interstitial cells are like HeLa cells, which I've heard about in my past life. They can divide and reproduce indefinitely, so cloning interstitial cells is the easiest thing to do. Wu Xian directly cloned a large number of them for experimental storage.

Several months later.

Wu Xian breathed a sigh of relief.

He hadn't expected that even with his multiple shadow clone technique and super brain, it would take so long to complete just the first step. As a result, he had to clone and cultivate rare cell tissue samples.

Otherwise, it simply cannot withstand the consumption.

Fortunately, it finally succeeded.

At this moment, gazing at the two bodies lying quietly in the center of the experimental table, a glimmer of relief finally flashed in Wu Xian's eyes. Success.

This first step, overcoming numerous barriers of resources, technology, and time, was finally accomplished by him.

They were two clones that bore a striking resemblance to his original form, with smooth muscle lines and stable vital signs, as if they had simply fallen into a deep sleep.

However, the power contained within them is enough to cause a huge upheaval in the ninja world.

The one on the left has skin with a warm, jade-like luster. This is the "Byakugan Shikotsumyaku Clone," which is a fusion of the secret cells of the Hyuga main family, the mutated and powerful Shikotsumyaku gene of Kaguya Kimimaro, and the cells from that special individual "Uchiha Shin" that have extremely strong compatibility and universal adhesive properties. Finally, it was carefully cultivated based on Wu Xian's own basic cells.

The one on the right is even more mysterious. Inside its body, a vast amount of life energy surges like a tide, a near-limitless vitality originating from the cells of the ninja god, Hashirama Senju.

What's even more striking are those eyes that have already opened—within a scarlet background, each eye has a jet-black magatama slowly rotating, emitting a cold and eerie light.

Sharingan!

Moreover, it was a Sharingan that was naturally awakened without experiencing strong emotional stimulation, solely through cell fusion and chakra nourishment!

The composition of this "Wood Release Sharingan Clone" is even more complex and terrifying: it not only integrates Hashirama's cells, but also combines the cells and Sharingan tissues of Itachi Uchiha, Shisui Uchiha, two unparalleled geniuses of the Uchiha clan, as well as more than a dozen Uchiha elites.

Shin Uchiha's cells once again played a crucial role, acting as the best harmonizer and catalyst, greatly promoting the fusion of Wood Release and Sharingan, two originally opposing forces, achieving an unprecedented, near-perfect balance.