Chapter 154

Chapter Making Devil Fruits (seeking subscription, fifth more)

“Chen Fan, I advise you to give up. You can’t get past the simple plant growth.”

“Enough technology.”

“You… awesome, when I didn’t say it, then you say, how do you want to do it?”

Chen Fan looked at everyone and said, “At this stage, I have hired people to cultivate land. It is processed in batches. There will only be more and more land nearby, and the forest will become more and more luxuriant. To achieve a balance, I will stop the use of fertilizer.”

Everyone can understand this. After all, if you continue to use it when the time comes, it will actually destroy nature.


“Gene modification, gene creation, I have completed the last step, and the rest is the mapping and combination of genes. I will share the data with you. Don’t worry.”

Chen Fan felt that he couldn’t hold on any longer. Genetic modification is really a big task. It has been off for three months. If it continues, Chen Fan’s temperament can’t stand it.

So I plan to deal with it in advance, so I can be assured.

Everyone thought about it, since Chen Fan has invited, he is ashamed to refuse.

Let’s chant, everyone is biologists, who-has no level yet?

Recombinant DNA molecules need to be replicated and amplified in recipient cells, so genetic engineering can also be characterized as molecular cloning (Molecular Cloning) or gene cloning (Gene Cloning).

In a broad sense, it includes hybridization techniques in traditional genetic manipulation, genetic engineering and cell engineering in modern genetic manipulation.

Refers to the industrial design and application of DNA recombination technology, including two major components: upstream technology and downstream technology.

Upstream technology: design and construction of gene recombination, cloning and expression (ie DNA recombination technology);

Downstream technology: large-scale cultivation of genetically engineered bacteria (cells), separation and purification of exogenous gene expression products.

The broad concept of genetic engineering is more inclined to the category of engineering.

Gene engineering in a broad sense is a high degree of unity:

The design of upstream recombinant DNA must be guided by simplifying downstream operating processes and equipment;

The downstream process is the embodiment and guarantee of the upstream reorganization blueprint. —The basic principles of the industrialization of genetic engineering.

One item of information was listed.

Everyone stuck to the standard given by Chen Fan, a step-by-step experimenter.

This is genetic modification, and what Chen Fan is doing now is to create genes.

Creation is the process from zero to one.

This is the most difficult. It is not difficult to reform Yiji, but it is very difficult to create out of thin air.

“In essence, the definition of genetic engineering emphasizes that a new combination of foreign DNA molecules is introduced into a new host organism for reproduction. This new combination of DNA molecules is designed and operated in accordance with engineering methods. This gives genetic engineering the ability to cross the barrier of natural species, overcomes inherent limitations between species, and expands and brings about the possibility of directed modification of organisms. This is the biggest feature of genetic engineering.

Genetic engineering involves artificially connecting genes from different organisms with vector DNA with autonomous replication capability in vitro to form new recombinant DNA, which is then sent to the recipient organism for expression, thereby resulting in the transfer and recombination of genetic material.

Elements of genetic engineering: including foreign DNA, carrier molecules, tool enzymes and recipient cells. ”

Chen Fan knocked on the blackboard, explaining the technology, everyone listened.



All I am doing are people who have impacted the Nobel Prize in Biology, but now listening to the new knowledge explained by Chen Fan, I feel that it is completely unconstrained.

But you have to admit that these are all understandable, this is the most ridiculous.

“If it’s almost the same, I suggest starting the gene assembly. You still have one and a half months to complete it. Each of me will give a house, and each person will be responsible for an area. I don’t want any area to go wrong.”

Who dare?

They are all big brothers, and whoever makes a mistake can’t afford to be ashamed.

Fuck, I’m all this old, and I was pulled by Chen Fan.

Zhang Dongshan said: “If there is nothing wrong, then start their own experiments. Chen Fan said that the house that Chen Fan gave to the house must be the house built on Chen Fan’s side. I think the house built by Chen Fan is not bad, right?”

0 ···Find flowers·····

Fuck, this is awesome.

Chen Fan was stunned for a moment, looked at Zhang Dongshan, fucking, when did I say to build a house?

You are pitting Laozi.

However, according to what Zhang Dongshan meant, he had to build a house. If he didn’t make a house, that would be unrealistic.

“I can build a house, but there is a premise.”

“you say.”

“I want to make something, something you all want me to make.”


Zhang Dongshan froze for a moment and said, “Chen Fan, are you sure? Invest in construction, don’t need money?”

“Yes, no money, but over there, I hope to build a conference hall for scientific researchers all over the world to use for meetings.”

.. .. …….

“This… Chen Fan, if you restore it, no one will stop you for what you are doing, but how much money do you need, have you thought about it?”

“I have thought about it, but I don’t think it will cost much. I think it can be successful.”

Time passed day by day.

“The most commonly used method for direct gene isolation is the “shotgun method”, also known as the “shotgun method.” The specific method of the shotgun method is to cut the DNA in the donor cell into many fragments with restriction enzymes, and load these fragments separately. Into the carrier, and then transferred to different recipient cells through the carrier, so that all fragments of the DNA (ie, foreign DNA) provided by the donor cell are replicated in large numbers in each recipient cell (called amplification in genetics) , Such as using PCR technology), find out the cells containing the target gene, and then use a certain method to isolate the DNA fragments with the target gene”

“Are you idiots? I also need to teach this?”

One by one, they are directing their students and carrying out their work.

Strict, speed.

Is the core.

After each stage is completed, Chen Fan will complete a patchwork.

The time has come to the end of May.

In June, ushered in the new college entrance examination.

At this time, the first fruit appeared in front of everyone.

“It’s not an exaggeration to call this a devil fruit.”

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