MYGO's guitarist has ice attribute bonus

"I hope you will think about it carefully and stop misunderstanding your feelings. This way... you can save yourself a lot of unnecessary trouble." After saying that, Hailing continued to move forward.

What are my emotions like?

Chu Hua lowered his head, lost in thought. "My feelings towards Xiao Ai are just those of a good friend, right? Just now, I just happened to see Xiao Ai like that... well, it was just a coincidence..."

Thinking of this, Chuhua followed again.

Chapter 294: News from Dr. Yamada

As the sun sets, the entire cherry blossom forest is dyed amber. Along the branches and leaves, the lazy and mellow light pours down on everyone's shoulders drop by drop.

Countless petals are almost transparent in the slanting light, emitting a golden halo, and swirling down in the breeze.

"This light is so beautiful, everyone, let's take a photo together?"

Aine stood under a tall cherry tree, facing the sunlight, and her entire outline was coated with a layer of gold.

This time, Deng, who was following Aine, was the first to speak up: "Taking a picture...with Aine-chan, yeah."

She really wanted to have such a beautiful photo with Aine.

"Okay." After Xiangzi set up the tripod and adjusted the camera parameters, she couldn't help but look at Aiyin a few more times through the viewfinder.

"I... want to stand next to Aine-chan." Mu gently pinched the corner of Aine's skirt, her fingers turning white from the force, and the cool touch of the silk fabric made her heart beat faster.

“Sure.” Aine nodded, a smile in her eyes. Then, she looked at the others and called out, “Everyone, come over and take pictures.”

Hearing Aine's cry, Riki and Lenai didn't say much, but just walked quietly to Aine's side.

"Want to take a photo? I haven't taken a photo with everyone yet." Miao Meng's eyes were obviously interested. She hurried to the camera, but because of her height, she could only stand behind it.

"It's really a rush." ​​Hailing couldn't help shaking her head when she saw Miao Meng's anxious look, but she also walked to the camera.

Aine glanced around and set her sights on Soyo, who looked hesitant. "Soyorin, come on, everyone's finally taking pictures."

Hearing this, Su Shi raised her head and met Aine's gaze, a hint of hesitation flashed in her eyes.

You'll always be with me, right? Then... there's no need to take pictures... Aine-chan... is that right?

Just as Su Shi was lost in thought, Aine's voice suddenly became louder, and she instantly came back to her senses. Before she knew it, Aine was already in front of her: "Soyorin? What are you thinking about? Come here quickly."

"Eh?"

Before Sushi could say anything, Aine gently grabbed her hand and slowly pulled her towards the camera.

"Wait, Aine-chan." Although Su Shi wanted to refuse, she didn't say the words of refusal when she saw Aine's back.

"Then..." Aiyin continued to count the people, "There's still one person missing, Chuhua."

As soon as Aiyin said this, Chuhua trotted over to them: "Xiaoai, I'm here, sorry."

Aine smiled slightly: "Nothing."

Seeing that everyone had arrived, Xiangzi set the countdown and ran in between them.

The camera started counting down, and the ten-second wait suddenly seemed incredibly long.

Sushi turned her face away. She stood to Aine's right and could smell the faint scent of cherry blossoms in Aine's hair, probably because of the cherry blossoms that had fallen on her hair earlier. When Aine unconsciously leaned towards her, her mind unconsciously accelerated.

Just as the shutter was about to click, a sudden spring breeze swept through, plunging thousands of cherry blossoms from their branches and sending cherry-pink snow falling from the sky.

"Click."

Time stood still at that moment. The photograph captured the moment when ten girls were surrounded by petals: Xiangzi held Mu, grinning broadly; Mu held Xiangzi with one hand and pinched Aine's skirt with the other, the corner of his mouth slightly raised; Deng stood beside Su Shi, his eyes fixed on Aine; Su Shi twisted her face, a look of reluctance on her face, but the faint smile at the corner of her mouth betrayed her true feelings; Miao Meng, Hai Ling, and Li Xi stared calmly at the camera; Le Na raised her head, as if attracted by the sky full of cherry blossoms; Chu Hua used the flying petals to mask her eyes.

Aiyin, who was in the middle, had her hands behind her back, looking at the camera with a smile on her face.

"I want to see it, I want to see it." Aine walked towards the camera, a few flower petals still stuck to the ends of her hair. The moment she saw the photo, she grinned, her canine teeth particularly eye-catching. "This is a really good photo, Soyorin, this one is much better than the first photo we took of mygo."

"Really..." Su Shi nodded.

As dusk deepened, the cherry blossoms began to emit their unique sweet fragrance after sunset. Streetlights gradually came on, casting mottled shadows on the falling petals.

"It's already this late." After developing the photos, Aine carefully placed them in her hands, "Everyone, the photos have been developed. There are ten in total."

After distributing the photos to everyone, Aine raised her right hand with some difficulty and gently waved goodbye: "It's almost time to go home, everyone, bye."

Of course, even though Aine said that, Xiangzi and the others didn't dare to let Aine go home alone.

……

Aine was lying at home. Some time ago, she, her mother, and Su Shi went to NCNP again. After the examination, she wanted to know the result of the application, but Dr. Yamada told her that the review was still underway.

"Hey, when can I get some news?" Aiyin turned over with great effort, and kept looking through the conversation between herself and Dr. Yamada.

But as she flipped through the pages, she saw a small bubble with the number "+1" at the bottom.

"Eh? Is this...new news?" Aine's eyes suddenly widened, and she quickly flipped to the bottom of the page.

[Dr. Yamada: Chihaya, the PMDA and IRB reviews have been passed.]

Looking at these words, Aine was stunned for a long time. Didn’t Doctor Yamada say... that this application would be difficult to approve?

Not long after, Aine slowly poked the screen with her finger.

[Aine: Dr. Yamada, that means?]

[Dr. Yamada: Yes, you are ready for surgery. We will discuss the treatment plan and surgical preparations at our hospital. Once the preparations are complete, I will notify you of the surgery time, and you can be admitted to the hospital at that time.]

[Aine: Okay.]

Putting the phone down, Aine stared blankly ahead, seemingly still recovering from the surprise. "I... can really have the surgery? I don't know if it will be successful, but... finally, I don't have to keep living like this... finally, I don't have to wait to die..."

After a long, foolish smile, Aiyin slowly climbed down from the bed and opened the door: "Mom..."

After hearing Aine's voice, Aine's mother ran out from the kitchen: "What's wrong, Aine?"

"Just now, Dr. Yamada sent a message saying that the review has been approved and the surgery can be performed..."

As she said this, Aine’s voice was shaking.

"Really?" Aiyin's mother's pupils shrank. At this moment, she felt a mixture of worry and joy, but she didn't show it. She knew that Aiyin definitely didn't want to see her worried look at that moment. "Then mom is very happy. Have you told Xiaoxiang and the others?"

"Not yet. I'll talk to them after I finish talking to you."

Girls' Prayers

Chapter 295 Surgery Discussion

NCNP, hospital conference room.

A dozen neurologists sat at a long table, each looking solemnly at Dr. Yamada standing on the podium.

This meeting was their first discussion on curing ALS. Of course, doctors from not only Japan but also other countries were very interested in this.

"I plan to first silence the expression of the SOD1 mutant gene in Chihaya's body more thoroughly, and then perform gene editing. What do you think?" Dr. Yamada showed everyone the PPT he had prepared, on which were the surgical steps he had designed.

"A more thorough silence? Doctor Yamada, I remember you've already used ASO to silence Chihaya-san before, right?"

"Indeed," Dr. Yamada nodded. "I used to use ASO for silent expression, but the effect wasn't very ideal."

"So...are you planning to use AAV instead of ASO?"

After these words were spoken, the other doctors' expressions became even more solemn.

"But, Dr. Yamada, if you really want to use AAV instead of ASO, can that little girl withstand it? After all... the risks of AAV are much higher than those of ASO."

"You're right. AAV does carry a higher risk than ASO," Dr. Yamada said without denying it. "However, to ensure the safety and effectiveness of gene editing, silencing the SOD1 mutant gene is essential with current technology."

At this point, Dr. Yamada once again recalled the day when he told Aine whether or not to choose early surgery. Originally, he was just fulfilling his obligations as a doctor, but... when he really saw the determination in Aine's eyes, his heart couldn't help but be moved.

I really want that child to recover. She is so young, but she has to go through such a cruel disease. I really... can't bear it.

"But, Dr. Yamada, I understand what you mean. You're also concerned about that girl, but...isn't the basis for our surgery to protect the patient's life? What if a cytokine storm, organ failure, or even sequelae really occurs? What should we do?"

Dr. Yamada's response was calm: "I will be prepared for these things."

"Doctor Yamada, I hope you can consider this carefully. This is not a joke. Currently... there are no cases of ALS being cured in the world. Everything we've done is based on theoretical research. If we were to waste such a young girl's youth because of this, it would be really..."

"I know." Dr. Yamada nodded. "Indeed, these are all theoretical studies."

"Also, the success rate of AAVs isn't very high right now. Can you achieve it without missing the target?"

Dr. Yamada remained silent on this question. What the other doctors said was indeed correct. Whether it was cytokine storm, sequelae or even off-target effects, these were all difficult problems facing us.

Dr. Yamada's eyes gradually became firm. "I know these are the current problems. Whether it's the cytokine storm you mentioned, or the sequelae, or even off-target effects, once they occur, the consequences may be difficult to reverse, but..."

Dr. Yamada lowered his head and forced a smile. "The child told me that she wanted to try. As long as there was a chance, she didn't want to wait for death."

"To cure ALS, AAV is necessary. With current technology, ASO is less effective at silencing SOD1 than AAV and is far less potent than AAV... Besides, I've already used ASO as an over-treatment, but the results aren't ideal. This is the only way."

After hearing what Dr. Yamada said, the other doctors fell silent. Dr. Yamada was right. If they wanted to cure ALS, then AAV was a hurdle that they could not avoid. They did not have the ability to develop an efficient ASO. If they wanted to see results, they could only pin their hopes on AAV, which might have side effects.

"I remember that girl's ALS was caused by a single mutation in the SOD1 gene, right, Dr. Yamada?"

"Yes." Dr. Yamada nodded.

"Dr. Yamada, if anything goes wrong during the surgery, the PMDA and IRB will definitely hold you responsible. Even so, have you thought this through?"

"Yes, I've thought it through." Dr. Yamada said decisively, "Ever since I told Chihaya that she could apply for early surgery, I've been mentally prepared."

"Is that so? Well... I'll help you too. I don't have much else to do right now. To be honest, this is my first time performing ALS surgery, so I'm a little nervous. So, I can only assist you. Dr. Yamada, you won't mind my clumsiness, will you?"

Hearing this, the other doctors couldn't help but laugh out loud, adding a touch of levity to the tense meeting room.

"Huh?" Dr. Yamada looked at the doctor in front of him. Obviously, what he said was a lie. As a well-known neurologist in NCNP, how could he have nothing to do?

Of course, Dr. Yamada also understood what he meant by this sentence.

Dr. Yamada smiled slightly. "Really? I wouldn't dare to disrespect a famous neurology doctor."

"That's good."

"Let me help too. After all, it would be a shame if I didn't participate in this kind of surgery."

"Me too……"

Dr. Yamada watched as the doctors continued to express their opinions, and his eyes couldn't help but become moist. "Really... then thank you all."

"You're welcome. We're all friends after all." The doctor who had just spoken out first pulled out his phone. "It just so happens that I have doctor friends in the United States and in the Eastern countries. I'll ask them about this."

"Dr. Yamada, what have you decided to use as the payload for AAV to silence gene expression?"

"I plan to use AAV9-dCas9-KRAB for silencing therapy."

After hearing Dr. Yamada's words, several doctors frowned slightly. "AAV9? Does that mean you want to use AAV as a vector in the future?"

"Yes," Dr. Yamada nodded in response. "After all, the gene editing surgery will require a vector, and AAVrh10 is a good candidate. The capsid protein structures of the two are quite different. If we produce antibodies after gene silencing, we can bypass the neutralizing effect of the pre-existing antibodies."

"But... the impact of one AAV is so huge, if there is another one... can that girl really be okay?"

"I've considered what you said." Dr. Yamada switched to the next page of the PowerPoint presentation. "If we use other vectors, the targeting will be even worse than AAV. In that case... we might have to undergo multiple surgeries. With AAV, at worst, two surgeries would be enough."

Hearing this, the other doctors nodded, saying that AAV's targeting ability is indeed relatively excellent among the vectors used to treat ALS.

However, there are also high costs and risks.

Chapter 296 Mygo's Tour

In RiNG, Aine tells Suyo, Deng and Riki what Dr. Yamada told her without reservation.

"I see, that's what Dr. Yamada said..." Su Shi rested her chin on one hand, her eyes fixed on the table, as if she was thinking about something. "This is his current preparation for surgery..."

“Even though the AAV is riskier, do we still have to use it…” Deng gently grabbed Aine’s hand, his eyes slightly red, and it seemed like tears would flow out in the next moment.

Touma didn't want to listen to what Aine told them.

As early as yesterday, after Aine received the news that she had passed the surgery review, Deng's mentality had changed a little. Before the review, she could still comfort herself, after all, the surgery had not been decided yet, and she could still spend a long time with Aine... But now, the result has come out, and how long she can spend with Aine has become a mystery.

Although Deng had been mentally prepared when Aine told them that she would have surgery in advance, when that day really approached, the pain and fear in her heart were inevitable.

"There's nothing we can do about it, Tomorin. I told Dr. Yamada that what I want is... a healthy self, not just lying in bed quietly waiting to die..."

Such heavy words fell on Deng's heart. She lowered her head and a tear fell from her eyes, but no one saw it.

Death?... I had clearly witnessed death with my own eyes, the falling of flowers, and now... I had to watch the person I had promised to be with for the rest of my life face death...

Even if the flowers fall, they can bloom again...will Aine-chan do the same?

Li Xi sat nearby and waited for Deng to finish speaking before speaking, "So, what Dr. Yamada has decided now is to silence your mutant gene first. Only after that can the formal surgery be performed, right?"

“Yes.” Aine nodded, then smiled and teased Rikki, “I didn’t expect Rikki, who often skips classes and sleeps in class, to understand all this…”

Li Xi turned his face away: "Huh, do you need to be so talkative?"

Do I really understand this? Not really.

Li Xi knew very well in her heart that she naturally did not understand these biological knowledge. As for why she said this, it was because Aine had just said it in her words.