Chapter 75 Gene Spray
Jiang Yan's number in the magic forum is No. 2. He has many questions and wants to ask Merlin directly on the forum.

As Meilin is the manager behind the forum and the discoverer of magic civilization, Jiang Yan hopes to get answers from Meilin.

"Mr. Jiang, how is the situation of the magic forum today? We are still reporting routinely, and I will return the information to the headquarters when the time comes." The staff asked after entering the office.

"I can only see my number. The number has been 2 and hasn't changed. I can't see the total number."

"There is still only Merlin's only post on the forum, and no one else has posted."

"I still haven't figured out how to post. Today is the same as usual."

"The ability brought by the magic brain has also not changed, and it still maintains a vague perception of the seeds."

"Now all abilities are in the state of a black box. They can be used but cannot explain the principle."

After Jiang Yan's brief report, the staff continued to ask:
"Sir, after reading "Zhouyi Cantongqi" and "Enlightenment Chapter", did the devil brain in your body react? Or did you have any insights?"

Both "Zhouyi Cantongqi" and "Enlightenment Chapter" belong to the Taoist works of practicing inner alchemy. The Bureau of Abnormality Investigation deliberately found a long history handed down for Jiang Yan to understand.

"I don't have any comprehension. These contents do not belong to the same system as magic at all. I don't think the cultivation method passed down from ancient times is the same as the magic brain."

Due to the emergence of magic, the Bureau of Abnormality Investigation not only collected various ancient collections in the country, but also sent people abroad to collect magic-related materials.

Some ancient magic-related cultural relics have been sold for sky-high prices, especially the ancient magic-related cultural relics unearthed in England.

It is speculated that Merlin is an Englishman, and the name Merlin itself is also a magician in English myths and legends, so England's magical artifacts are particularly favored by insiders.

After finishing the routine question and answer session of the day, Jiang Yan continued to think, he found it very strange and even a little funny.

Merlin said in the post that the magic forum was created for everyone to communicate, claiming that it is to let everyone realize the value of thinking, and it is a platform for communication to show value, but he did not see communication or communication.

It's as if the group owner said that everyone is welcome to speak, and then set a ban on all members.

Jiang Yan was a little puzzled, because he got the news that he was not the only one who got the magic brain in the world, Huaguo speculated that Enceladus was registered and there was at least one magic coin in the world.

At present, Huaguo has two guesses, either the other magic coin has not been used yet, and people can speak on the magic forum, but Jiang Yan hasn't found a way yet.

Another guess is that another magic coin was used. The reason why the magic forum cannot post is that some conditions have not been met.

At present, regarding magic, American has a very tight embrace with England, while Huaguo chooses to share limited information with Russia. There is currently no communication mechanism on this topic.

"Mr. Jiang, we actually don't need to select seeds in this way. It is difficult for the selected seeds to achieve the desired effect, and it is impossible to form a sufficient variable control group."

"The seeds bred in this way have different mutation points. It is difficult for you to draw conclusions through controlled experiments. We cannot publish papers like this." After another week of breeding time, Dong Wenxu reminded again.

He went on to say:

"If you want to pursue large-scale cultivation of genetically mutated seeds and feel that the current conventional method is too inefficient, we can use carbon-dot spray for gene editing."

"Carbon dots formed from spherical natural materials smaller than 10nm nanoparticles can serve as fast vehicles for carrying plasmids into mature plant cells."

"Thereby leading to transient plant transformation of species in many important crop species without negative effects on photosynthesis or growth."

"Introduction of GFP, Cas9, and gRNA-expressing gene fragments in wheat by foliar application (spraying) of plasmid-coated carbon dots and, in the case of Cas9, genome editing in the SPO11 gene."

"This spray-on gene editing protocol is simple, cheap, fast, and applicable to a variety of crops."

"And it is more targeted, which is convenient for subsequent controlled experiments."

Nowadays, in the subdivided field of biology, breeding, more cutting-edge research methods use directional gene differentiation to study the differences between assumptions and actual conditions.

When looking at mutation rates conferring herbicide resistance in natural plant populations, and examining whether mutation rates increase when plants are stressed by sublethal herbicide exposure.

The scientists used the cereal amaranth and resistance to acetolactate synthase (ALS)-inhibiting herbicides as model systems for the discovery of spontaneous herbicide-resistant mutants, screening a total of nearly 8000 million plants.

As for Jiang Yan's research method, not to mention how far behind the most advanced ones, it belongs to the research method of Shennong tasting hundreds of herbs in ancient times.

Jiang Yan knew that something needed to be done to stop Dong Wenxu from thinking too much: "This method was developed by Thomas from Bristol and Keith from the School of Chemistry in 19."

"I am more familiar with this than you. The problem with this method is that it cannot guarantee that the gene editing you want will happen as expected."

"You can't guarantee that the plant will be transformed after spraying the fast vector. In the real experiment, there is such a probability. How do you select the seeds that the real gene induces differentiation?"

"Don't you still have to go through conventional experiments? This method is not superior to the current mainstream experimental methods. It looks faster, but it's just that you feel faster."

"The screening means of this method are more cumbersome than conventional ones."

"If this method is really as superior as Thomas wrote in his article, you can see it being promoted on a large scale in the Institute of Agricultural Sciences."

"I know you don't believe that I can screen out better seeds based on my feelings. I think that even if I can screen out better seeds, I can't publish papers with enough impact factors without reference experiments."

Dong Wenxu is stupid. He has only read about this method in articles, and has never actually practiced it, let alone pointed out its disadvantages.

What shocked him even more was that he was really impressed after reading this article, and he took it out to change Jiang Yan's research method a little bit, but he didn't expect the other party to blurt out the ins and outs of this paper.

Even the author of the paper, the university, and when it was published are all clear.

Dong Wenxu murmured: "Without papers, how can we judge whether our work is good or bad? In addition, if we can really screen out better seeds in this way, do we still need the existence of breeding science?"

 Thanks to zyi2 and Ironboy3000 for their plans!
  I wish all book friends a happy Year of the Tiger and a happy Chinese New Year (*^_^*)
  
 
(End of this chapter)

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