As a mage, I just want to pursue the truth

Chapter 76 Dawn
"In agriculture, a paper is just a summary of your work. The essence of evaluating your work is the positive impact your research results have on agriculture. Publishing a paper is just a side effect of the essence."

"I have no way to convince you of my method now. The maturity period of cabbage is about forty days. If you take good care of the crops, the final result will prove that I am right."

Dong Wenxu didn't know where Jiang Yan's full confidence came from, he only hoped that the work during this time was not useless.

However, in the process of planting, Dong Wenxu can experience the fun from it, and sometimes he thinks that the Hua people's farming genes are too strong.

Farming is an instinct deeply rooted in the genes of Chinese people. Jiang Yan continued to devote himself to agricultural research after obtaining the standard magic brain.

Jiang Yan can perceive life, so Simmons also has this ability, and the characteristics given by the standard magic brain are the same.

Simmons joined Pfizer's special drug research and development team, and American hoped that he could play a magical role.

The researchers identified twelve proteins that were identified as potential targets.

It can be roughly divided into two categories: the first is structural proteins, including spike proteins, envelope small membrane proteins, membrane proteins, nucleocapsid proteins, and another type of proteins called nonstructural proteins.

The non-structural proteins mainly include protease (M pro), papain, nsp13: helicase, nsp12: RNA-dependent RNA polymerase, nsp14: N-terminal exoribonuclease and C-terminal guanine-N7 methyltransferase, nsp15: urine Nucleotide-specific endoribonuclease, nsp16:2'-O-methyltransferase and nsp10.

Viruses are responsible for the replication and packaging of viral core material into a capsid protein shell.

Blocking any of these proteins leads to a stall in the virus' replication or packaging of its RNA into a protein coat.

Among a total of 12 proteins identified as targets for controlling CoV; 3CL pro was identified as one of the most important targets due to its critical role in mediating viral replication and transcription.

3CL pro is a chymotrypsin-like cysteine ​​protease (~33 kDa) that has been similarly called a major protease due to its dominant role in processing replicase polyproteins and genes.

Although 3CL pro is a dimer that provides a substrate-binding cleft, the protease exerts its proteolytic activity through a cysteine ​​thiol-directed nucleophilic attack in its Cys-His bimolecule.

The viability of 3CL pro during the viral life cycle is lacking evidence for its human analogue.

Therefore, Pfizer researchers only think that 3CL pro may be used as a potential main drug target to bind and inhibit viral replication activity.

They need Simmons to judge from his perspective.

There is a high similarity between the 3CL pro structure and the 3CL pro of different virus mutants, and inhibitors previously designed for MERS or SARS can also be effective against the current epidemic virus.

At the same time, an inhibitor called N2, previously designed to inhibit 3CLSARS-CoV and MERS-CoV, was also involved in the test against the virus this time.

Some investigators have proposed targeting 3CL pro, where the efficacy of available drugs such as ritonavir, emetine, hesperidin, lopinavir, and indinavir have shown potential to inhibit 3CL pro.

Green tea phytochemicals have also been intensively evaluated to identify phytochemicals with potential to inhibit 3CL pro.

In addition, neem phytochemicals were evaluated against the target proteins of SARS-CoV-2, membrane protein (M) and envelope protein (E), among which Nimocin, 24-Methylenecycloartan-3-one, phytosterols, Beta-Amyrin and Nimbolin A were injected into mice by Pfizer researchers.

Simmons touched the mice one by one. He didn't know if it was his psychological effect or it was true. He thought that touching them with his own hands would give him a better judgment.

"I think it's just the best guinea pig out there."

The researchers beside him hurriedly disinfected him, and another researcher asked, "This mouse was injected with a protease inhibitor."

"We developed protease inhibitors based on five popular M pro variants in the new coronavirus lineage, including G15S, T21I, L89F, K90R and L205V."

"I think we need more observations. We have just completed the initial injection. It is still unknown whether the mice injected with protease inhibitors can still maintain the best condition."

　I wish you all a happy new year and all the best (*^_^*)
　　
　
(End of this chapter)