godfather of surgery

Chapter 1049 Castration Virus

The Virus Laboratory of Nandu Medical University is a Level 3 biosafety laboratory, also known as a P laboratory.

According to the infectiousness and harmfulness of pathogenic microorganisms, biosafety laboratories are divided into four biosafety levels internationally: P1, P2, P3 and P4. The so-called P4 laboratory is the laboratory with the highest biosafety level currently owned by humans and is known as the "aircraft carrier" in the field of virology research.

The P4 laboratory of Nandu Medical University is under construction. Once completed, it will be the second P4 laboratory in China.

A week later, visible turbidity had appeared in the sterile transparent culture fluid in the laboratory. When the researchers in protective clothing saw the turbidity, they were extremely excited.

These turbidities are nothing but the cultivated K virus complex. Yang Ping temporarily calls the combination of his virus and K factor the K virus complex.

The researchers used special tools to carefully remove a portion of the liquid containing the virus. Although these viruses were not contagious and had been castrated and were unable to replicate, they still strictly followed the standard experimental operation procedures and did not dare to be negligent in the slightest.

These samples will be subjected to the most rigorous testing to determine whether they are pure, whether they have mutated, or whether they are mixed with other harmful microorganisms.

Viruses are microorganisms that have the ability to replicate themselves, so they must be treated with caution. Of course, as long as these viruses do not undergo rare mutations, they are still safe and do not pose any danger to the human body. At most, they may cause some transient mild flu symptoms, and these symptoms are self-healing and will not leave any sequelae after healing. This is why some viruses can be used to treat tumors.

Some samples were specially treated and then sent to an electron microscope for observation in order to elucidate their structure.

The basic unit of the structure and function of life is the cell, but the virus is an exception. It is a non-cellular life and does not have a cellular structure. It is just a simple protein structure. Of course, the simplicity here is only relative to the complexity of cellular organisms.

Because viruses have no cellular structure, they must parasitize living cells in order to survive and reproduce. They cannot live independently and must rely on host cells to provide the necessary conditions for life activities. They reproduce within host cells through processes such as adsorption, injection, replication, combination and release.

Since birth, humans have had to deal with countless viruses, bacteria and other microorganisms. After a long period of evolution and development, some microorganisms have become part of our bodies.

For example, the organelles in human cells - mitochondria.

Mitochondria are organelles in almost all eukaryotic cells. In ancient times, they were originally free-living viruses that could produce energy in the presence of oxygen. They were then engulfed by ancestral eukaryotic cells. When these viruses parasitized on the ancestral eukaryotic cells for too long, they slowly forgot their original identity and became an indispensable energy generator in the cells - mitochondria, which is the site of cellular respiration and many important metabolic processes.

Professor Zhang Zhiwei is an outstanding virologist who was invited back from the United States by President Rao. At that time, Professor Zhang returned to China as part of the Thousand Talents Plan and came to Nandu Medical University to lead virology research, pushing Chinese virology research to the world's top position.

He was just a virologist and didn't know much about using viruses to treat tumors. But when he heard that Yang Ping was going to use the new virus he had cultivated directly in human experiments, he couldn't help but feel a little worried, because this was against the principles of scientific research ethics. If something really went wrong, the consequences would be disastrous and everyone would be implicated.

When Professor Zhang heard that Yang Ping from Sanbo Hospital wanted to cooperate with him, he was very happy at first. But after understanding the situation, he felt uneasy. This kind of thing should be avoided if possible. It is better not to challenge some ethical rules casually, otherwise it is easy to put oneself in an irretrievable situation. There is no need to take such risks.

However, after talking with Yang Ping face to face, Professor Zhang changed his mind and began to admire and respect Yang Ping. Yang Ping's selfless courage for science made him ashamed, so he finally agreed to cooperate.

However, for safety reasons, Professor Zhang imposed extremely strict requirements on the team's scientific researchers on the details of the experiment. Researchers without three years of virus research experience were prohibited from participating in the experiment.

Although these viruses are relatively safe, they have been modified by Yang Ping. Yang Ping combined the virus with the K factor to form a brand new virus. Although this virus still maintains the structure and function of the original virus in terms of its main structure, the function of the embedded K factor is very single and only targets one type of tumor cells.

Professor He Zhiwei is not worried about what bad the virus will do, he is worried about the K factor. This protein structure did not exist in the world before. It was cultivated out of thin air by Yang Ping in the laboratory. It is not a virus, but just a protein molecule. It does not have the ability to replicate itself. So it cannot cause much trouble.

But this K factor is different after it combines with the virus. Although it is not the virus, it has now become a part of the virus, which means that it can now split along with the virus. This is very scary.

Factor K can activate the apoptosis program of tumor cells, causing the death of tumor cells. If it gets out of control, it can activate the apoptosis program of normal cells. After being injected into the human body, it indiscriminately attacks various cells of the human body, including cells of important organs such as the brain, heart, lungs, liver, and kidneys. Large-scale cell death will quickly cause organ failure and the patient will die.

For this reason, Yang Ping also considered this issue when designing the experimental plan. In order to prevent the experiment from getting out of control, he castrated these viruses, that is, castrated their replication ability. In this way, no matter how destructive they are, there is nothing to be afraid of, because they cannot divide, so there are only as many as there are, and their combat effectiveness is extremely limited. Moreover, no matter how destructive these viruses are, they will inevitably be destroyed.

In the laboratory, a small amount can be injected into the human body first. If unexpected damage occurs, stop the injection and immediately give symptomatic treatment. The virus will slowly die in the human body, which is much safer.

The technology of castrating the virus's ability to replicate is very mature. For example, this technology is used in the manufacture of vaccines, such as viral vector vaccines.

It is a vaccine prepared by using a virus (such as adenovirus) as a vector. The genes required for replication in these vector viruses are knocked out in advance, so that they can infect human cells but cannot replicate. The gene fragment corresponding to the specific protein of the target pathogen is inserted into the vector virus genome, and the prepared vaccine is injected into the human body to infect the body cells and express specific proteins in the cells. This causes both humoral immunity and cellular immunity, giving the body a higher immunogenicity.

This process is actually similar to the principle of Yang Ping's current experiment, so sometimes cross-disciplinary integration can create something new. Some things that were originally used in another field may play a huge new role when applied in a new field. The first batch of experimental viruses have used genetic recombination technology to knock out the genes required for replication, and have undergone "sterilization surgery" on them, so even if they have extremely rare mutations, they cannot pass on the mutations, and the mutated individuals will die soon.

Professor Zhang Zhiwei personally participated in the experiment. He observed the sampled virus samples using an electron microscope and found no mutations in the virus, indicating that the purity met the requirements of the experiment.

Speaking of virus mutations, not all mutations are bad for us. Very rare mutations can be lethal, but they can also be beneficial. However, most mutations have neither adverse nor beneficial effects, and some mutations do not even cause any changes. These are so-called "silent mutations."

There are many reasons for virus mutation, including replication errors, interaction with the host, and external conditions such as ultraviolet rays. At present, the main cause of mutation in the laboratory is replication errors. After being injected into the human body, mutations will also occur based on the interaction with the human body. However, these viruses are castrated versions, so even if they mutate, the impact is not significant.

Professor Zhang Zhiwei successfully obtained a certain number of samples, which after testing temporarily met the experimental standards. He couldn't wait to tell Yang Ping the good news.

After receiving the news, Yang Ping rushed to the laboratory of Nandu Medical University as quickly as possible. He wanted to see these K virus complexes with his own eyes to see what they looked like. Yang Ping made great efforts for them.

If everything goes well, he will then make the virus into an injectable solution, and then use a cold chain transport similar to that of a vaccine to deliver them to the Institute of Surgery. In the ward, Yang Ping will inject these new viruses into Sisi's body, and closely observe her after the injection.

"I have been studying viruses for many years and I know that vaccines often use viruses as delivery vehicles, but I never thought that this thing can also treat tumors?" Professor Zhang Zhiwei couldn't help but feel a little regretful.

Yang Ping told him: "I discovered the K factor first, and then chose to use the virus as a delivery vehicle. If there is no K factor, the virus as a delivery vehicle is meaningless."

Professor Zhang Zhiwei thought about it and felt that what Yang Ping said was very correct. In fact, the focus of his research was not on viruses as delivery vehicles, which was an old method that had been used in many fields. The focus was on the K factor. The most technically demanding aspect of the K factor was its ability to initiate the apoptosis program of tumor cells and kill tumor cells. This was the biggest discovery and could be said to be a revolutionary discovery in the field of oncology.

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"Make these viruses into injectable biological agents as soon as possible and send them to our department. I want to start human experiments as soon as possible." Yang Ping confirmed that these viruses met the experimental requirements.

"Does it have to be so urgent?" Professor Zhang Zhiwei was worried. He always felt that things were too rushed and there was something wrong somewhere. He felt uneasy, but he couldn't figure out which link had the problem.

"Yes, my patients don't have time to wait too long, I have to race against time." Yang Ping told him truthfully.

Professor Zhang Zhiwei said doubtfully, "I really want to know what kind of patient is worth taking such a risk. In fact, I personally think that you can just follow the routine and not be impatient. Even if you miss this patient, there are many similar cases. This one is not a big deal."

Yang Ping did not give any further explanation, and Professor Zhang Zhiwei stopped asking questions.
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Director Zhu completed his revenge. He did not leave China for Singapore immediately. He stayed to attend Yang Ping's wedding.

The overseas headquarters of An Ning Group is in Singapore. Since he was assigned to Singapore, he has been living in Singapore with his family. His children also go to school in Singapore. His family has settled in Singapore.

His battle with Japanese insurance companies finally came to an end. He defeated the Japanese and firmly occupied the Southeast Asian market. Now he is ready to move into Europe and the United States and truly go global.

So from the perspective of the company's development, this battle with the Japanese was a battle of life and death. He did not disappoint everyone's expectations, successfully defeated the Japanese, and won the victory in this fierce business war.

As for his personal vengeance, he also successfully completed a wonderful revenge. After so many years, he finally got justice for himself. Thinking of his experiences over the years, he was filled with emotion and felt a little uncomfortable, but he was very satisfied with his life now.

The way the Provincial People's Hospital handled his case was very wrong. They failed to withstand the pressure of public opinion and fired Director Zhu even though they knew that he was wrongly accused and framed.

Those hospital leaders did this just to smooth things over in order to keep their own official positions. They were doctors by training, so how could they not know that it was a colostomy operation? How could they not know that the reporters were intentionally slandering Director Zhu? However, they pretended to be asleep at that time. In order to put out the fire as quickly as possible, they rudely fired Director Zhu to deal with public opinion, which made Director Zhu feel very disappointed.

So since being fired that year, Director Zhu never set foot in the Provincial People's Hospital again.

But now he is going back. On behalf of An Ning Insurance Company, he donated 200 million yuan to the Provincial People's Hospital for the construction of advantageous disciplines. An Ning Group is very generous and has donated money to several first-line hospitals in Nandu Province.

Director Zhu’s current annual salary is not only tens of millions of RMB, but he also has shares in the company. Together with his annual salary and dividends, his annual income is already over 100 million RMB. So now he can be said to be one of the important figures in China’s insurance industry, and the first person to truly promote Chinese insurance overseas.

Now Director Zhu has returned to the Provincial People's Hospital, which can be regarded as a return to his hometown with wealth. He originally wanted to meet the dean who fired him, but unfortunately he did not get to meet him. The dean has been in prison for two years due to financial problems. He did not expect that such a big change would happen after a few years. He suddenly felt that a lot had changed.

He returned to his original department. The old director had retired and the current director was a brother of about the same age. He looked at the ward which had not changed much. The doctor's office and the duty room were still in the same locations, and even the beds had not been changed. He couldn't help but feel a lot of emotion.